In Vitro Toxicity Evaluation of 25-nm Anatase TiO2 Nanoparticles in Immortalized Keratinocyte Cells

被引：21

作者：

Chan, Jin ^{[1
]}

Ying, Tang ^{[1
]}

Guang, Yang F. ^{[1
]}

Lin, Li X. ^{[1
]}

Kai, Tang ^{[1
]}

Fang, Zhang Y. ^{[1
]}

Ting, Ye X. ^{[1
]}

Xing, Lin F. ^{[1
]}

Ji, Yang Y. ^{[1
]}

机构：

[1] Second Mil Med Univ, Inst Biophys, Shanghai 200433, Peoples R China

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 2011年 / 144卷 / 1-3期

关键词：

TiO2; NPs; Reactive oxygen species; Cellular toxicity; Transmission electron microscopy (TEM); Cytoskeleton; ULTRAFINE PARTICLES; CELLULAR UPTAKE; MANUFACTURED NANOPARTICLES; MAGNETIC NANOPARTICLES; CARBON NANOTUBES; CYTOTOXICITY; NANOTECHNOLOGY; SIZE; AGGLOMERATION; ENDOCYTOSIS;

D O I：

10.1007/s12011-011-9064-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Titanium dioxide (TiO2) nanoparticles (NPs) are massively fabricated and widely used in daily life, and thus potential risk has been posed to human health. However, the mechanism of the interaction between TiO2 NPs and cells is still unclear. In this study, the interaction of anatase TiO2 NPs with HaCaT cells is studied in vitro with multi-techniques. The TiO2 NPs not only insert into cells through endocytic pathway but also penetrate into the cell. The TiO2 NPs could produce reactive oxygen species (ROS) after dispersion spontaneously. Furthermore, the interaction between TiO2 NPs and cellular components might also generate ROS. The ROS generation could lead to cellular toxicity if the level of ROS production overwhelms the antioxidant defense. Cytoskeletal components, particularly the microfilaments and microtubules, cause modifications upon exposure to TiO2 NPs. With all results, the toxicological effects of TiO2 NPs on HaCaT cell can be simplified into six events.

引用

页码：183 / 196

页数：14

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