The comet assay for the evaluation of the genetic hazard of pollutants in cetaceans: Preliminary results on the genotoxic effects of methyl-mercury on the bottle-nosed dolphin (Tursiops truncatus) lymphocytes in vitro

被引：56
作者：
Betti, C
Nigro, M
机构：
[1] UNIV PISA, DIPARTIMENTO BIOMED SPERIMENTALE, SEZ BIOL & GENET, I-56126 PISA, ITALY
[2] UNIV PISA, DIPARTIMENTO SCI AMBIENTE & TERR, I-56100 PISA, ITALY
来源：
MARINE POLLUTION BULLETIN | 1996年 / 32卷 / 07期
关键词：
D O I：
10.1016/0025-326X(96)84573-5
中图分类号：
X [环境科学、安全科学];
学科分类号：
08 ; 0830 ;
摘要：
In this study the single cell microgel electrophoresis (Comet assay) was used to evaluate the genetic effects of methyl-mercury in dolphin (Tursiops truncatns) lymphocytes in the dose range 1-8 mu g ml(-1). The results of in vitro exposure showed that methyl-Hg induces DNA single-strand breaks and cytotoxicity in a dose-dependent manner. Dolphin lymphocytes have greater resistance to the genotoxic and cytotoxic effects of methyl-Hg than human and rat cells. The ecological significance of these results are discussed. Copyright (C) 1996 Elsevier Science Ltd
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页码：545 / 548
页数：4
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