Rapid Polymyxin/Pseudomonas NP test for rapid detection of polymyxin susceptibility/resistance in Pseudomonas aeruginosa

被引：16
作者：
Sadek, Mustafa [1 ]
Tinguely, Camille [1 ]
Poirel, Laurent [1 ,2 ,3 ]
Nordmann, Patrice [1 ,2 ,3 ,4 ,5 ]
机构：
[1] Univ Fribourg, Fac Sci & Med, Med & Mol Microbiol, Chemin Musee 18, CH-1700 Fribourg, Switzerland
[2] Univ Fribourg, INSERM European Unit IAME, Fribourg, Switzerland
[3] Univ Fribourg, Swiss Natl Reference Ctr Emerging Antibiot Resist, Fribourg, Switzerland
[4] Univ Lausanne, Inst Microbiol, Lausanne, Switzerland
[5] Univ Hosp Ctr, Lausanne, Switzerland
来源：
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES | 2020年 / 39卷 / 09期
基金：
瑞士国家科学基金会;
关键词：
Colistin; Rapid diagnostic test; Pseudomonas aeruginosa; Susceptibility testing; RESISTANCE;
D O I：
10.1007/s10096-020-03884-x
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Polymyxins are becoming an added value for treating infections due to multidrug-resistant Pseudomonas aeruginosa. Therefore, the Rapid Polymyxin/Pseudomonas NP test was developed for the identification of polymyxin resistance in P. aeruginosa isolates. This test detects the bacterial growth in a medium containing a defined concentration of colistin. Formation of basic metabolites is visually detected by yellow to purple/violet color change of the bromocresol purple pH indicator. The test performance was evaluated by using 50 colistin-resistant and colistin-susceptible P. aeruginosa isolates, among which 10 were colistin resistant. The sensitivity and specificity of the test were found to be 100% (CI95 65.5-100%) and 95% (CI95 81.8-99.1%), respectively, by comparison with broth microdilution as the reference method. The Rapid Polymyxin/Pseudomonas NP test is easy to perform, specific and sensitive, allowing rapid visual observation of results without the requirement any special reading equipment, and results are obtained in 3 h.
引用
收藏
页码：1657 / 1662
页数：6
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