Fucoidan inhibits UVB-induced MMP-1 expression in human skin fibroblasts

被引：81

作者：

Moon, Hee Jung ^{[1
,2
]}

Lee, Soon Rye ^{[1
,2
]}

Shim, Sun Nyu ^{[1
,2
]}

Jeong, Seok Hoon ^{[3
]}

Stonik, Valentine A. ^{[4
]}

Rasskazov, Valery A. ^{[4
]}

Zvyagintseva, Tatyana ^{[4
]}

Lee, Yong Hwan ^{[1
,2
]}

机构：

[1] Kosin Univ, Inst Natl Prod Hlth Promot, Coll Med, Pusan 602702, South Korea

[2] Kosin Univ, Coll Med, Dept Prevent Med, Pusan 602702, South Korea

[3] Kosin Univ, Coll Med, Dept Lab Med, Pusan 602702, South Korea

[4] Russian Acad Sci, Pacific Inst Bioorgan Chem, Far E Branch, Vladivostok 22, Russia

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2008年 / 31卷 / 02期

关键词：

matrix metalloprotemase-1; fucoidan; ultraviolet B;

D O I：

10.1248/bpb.31.284

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Ultraviolet (UV)B irradiation induces the production of matrix metalloproteinases (MMPs) by activating cellular signaling transduction pathways, which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. Using the human skin fibroblast (HS68) cell line in the present study, we investigated the inhibitory effects of fucoidan on MMP-1 expression by various in vitro experiments and elucidated the pathways of inhibition. Pretreatment with fucoidan inhibited UVB-induced MMP-1 expression in a dose-dependent manner. Extracellular signal regulated kinase (ERK) activation was markedly inhibited by treatment with fucoidan, though JNK activation was very slightly affected by fucoidan. We also found that fucoidan pretreatment significantly reduced MMP-1 mRNA expression in comparison with UVB irradiation only. In conclusion, our results demonstrate that fucoidan can mainly inhibit UVB-induced MMP-1 expression by inhibiting the ERK pathways. Therefore, fucoidan might be used as a potential agent for the prevention and treatment of skin photoaging.

引用

页码：284 / 289

页数：6

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