Dynamic quantitative phase imaging for biological objects using a pixelated phase mask

被引：65
作者：
Creath, Katherine [1 ,2 ,3 ]
Goldstein, Goldie [1 ,2 ]
机构：
[1] 4D Technol Corp, Tucson, AZ 85706 USA
[2] Univ Arizona, Coll Opt Sci, Tucson, AZ 85721 USA
[3] Optineering, Tucson, AZ 85719 USA
来源：
BIOMEDICAL OPTICS EXPRESS | 2012年 / 3卷 / 11期
关键词：
SHIFTING INTERFEROMETRY; MICROPOLARIZER ARRAY; COHERENCE; ERROR; FIELD;
D O I：
10.1364/BOE.3.002866
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper describes research in developing a dynamic quantitative phase imaging microscope providing instantaneous measurements of dynamic motions within and among live cells without labels or contrast agents. It utilizes a pixelated phase mask enabling simultaneous measurement of multiple interference patterns derived using the polarization properties of light to track dynamic motions and morphological changes. Optical path difference (OPD) and optical thickness (OT) data are obtained from phase images. Two different processing routines are presented to remove background surface shape to enable quantification of changes in cell position and volume over time. Data from a number of different moving biological organisms and cell cultures are presented. (C) 2012 Optical Society of America
引用
收藏
页码：2866 / 2880
页数：15
相关论文
共 46 条
  • [1] Competence between spatial and temporal coherence in full field optical coherence tomography and interference microscopy
    Abdulhalim, I.
    [J]. JOURNAL OF OPTICS A-PURE AND APPLIED OPTICS, 2006, 8 (11): : 952 - 958
  • [2] [Anonymous], 2012, POND LIF ID KIT
  • [3] [Anonymous], 2011, STEP HEIGHT STAND QU
  • [4] Born M., 1999, OPTICS
  • [5] Brock Neal J., 2007, U.S. patent, Patent No. [US7230717B2, 7230717]
  • [6] ABSOLUTE MEASUREMENT OF SURFACE-ROUGHNESS
    CREATH, K
    WYANT, JC
    [J]. APPLIED OPTICS, 1990, 29 (26): : 3823 - 3827
  • [7] Creath K., 1988, Progress in optics. Vol.XXVI, P349, DOI 10.1016/S0079-6638(08)70178-1
  • [8] Creath K., 2010, P SOC PHOTO-OPT INS, V7782
  • [9] Creath K., 2012, P SOC PHOTO-OPT INS, V8227
  • [10] Creath K., 2011, P SOC PHOTO-OPT INS, V7904
12345