Stability-Indicating RP-HPLC Assay for Simultaneous Determination of Chlorpheniramine Maleate and Prednisolone in Veterinary Injection

The present work described a simple, rapid, sensitive, accurate, and precise method for simultaneous determination of chlorpheniramine maleate (CHRM) and prednisolone acetate (PRED) in injection samples by high-performance liquid chromatography (HPLC) coupled with UV-Vis detection. Chromatographic separation was accomplished, employing isocratic mode and a mobile phase comprised of acetonitrile and a phosphate buffer (50:50, v/v, 30 degrees C), adjusted to pH 3.0. The flow rate used was 1.0 mL/min on a Thermo Hypersil ODS C18 colunm (5 mu m, 4.6 x 250 mm), and the injection volume of sample was 20 mu L. Analysis of CHRM and PRED was performed at a wavelength of 254 urn. The runtime for analysis was 12.5 min, and the retention times of CHRM and PRED were found to he 2.81 and 5.07 min, respectively. The calibration graph showed linearity over the concentration range 1070 mu g/ML for CHRM and 20-140 mu g/mL for PRED with a coefficient of determination (R-2) >= 0.9986. Repeatability mid reproducibility (expressed as % RSD) were lower than 1.72 mid 1.47%, respectively. The proposed HPLC method was demonstrated to he simple and rapid for the determination of CHRM and PRED in injection formulation, providing recoveries between 101.6-102.3%, whereas complete separation of degradation products, from analyte under investigation, provided the specificity of the proposed HPLC method.