Biocatalyst: Phytase Production in Solid State Fermentation by OVAT Strategy

被引：14

作者：

Soman, Somy ^{[1
]}

Kumarasamy, Suresh ^{[1
]}

Narayanan, Mathiyazhagan ^{[1
]}

Ranganathan, Muthusamy ^{[1
]}

机构：

[1] MGR Coll, PG & Res Ctr Biotechnol, Hosur 635130, Tamil Nadu, India

来源：

BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY | 2020年 / 10卷 / 05期

关键词：

Yeast; jack fruit seed powder; PSM medium; Ramachandran plot; Pichia membranifaciens; one source variable at a time; OPTIMIZATION; PURIFICATION; GROWTH;

D O I：

10.33263/BRIAC105.61196127

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The phytase-producing Pichia membranifaciens S3 (MG663581) was isolated from sugarcane juice using phytase screening medium. The predicted and experimental model showed maximum phytase production of 364 U/ml appearing g/100ml: Jack fruit seed (1.5), peptone (0.15), dextrose (0.50), yeast extract (0.05), malt extract (0.05) pH 5.5 and 280C) used OVAT strategy by Solid state fermentation through shake flask methodology. The modelling, 3D structure of Phy S3 amino acid sequences was modelled (PhyS3.B99990003) by using Modeler 9.23 and validated results showed that 86.4% in the favoured region by Ramachandran plot.

引用

页码：6119 / 6127

页数：9

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