Posttranslationally Modified Peptides Efficiently Mimicking Neoantigen: A Challenge for Theragnostics of Autoimmune Diseases

被引：23

作者：

Nuti, Francesca ^{[2
]}

Peroni, Elisa ^{[3
]}

Real-Fernandez, Feliciana ^{[2
]}

Bonache, M. Angeles ^{[2
]}

Le Chevalier-Isaad, Alexandra ^{[2
]}

Chelli, Mario ^{[2
]}

Lubin-Germain, Nadege ^{[3
]}

Uziel, Jacques ^{[3
]}

Rovero, Paolo ^{[4
]}

Lolli, Francesco ^{[5
,6
]}

Papini, Anna Maria ^{[1
,2
,3
]}

机构：

[1] Univ Florence, Lab Peptide & Prot Chem & Biol, Dept Chem, I-50019 Sesto Fiorentino, FI, Italy

[2] Univ Florence, CNR ICCOM, I-50019 Sesto Fiorentino, FI, Italy

[3] Univ Cergy Pontoise, Lab SOSCO EA 4505, F-95031 Cergy Pontoise, France

[4] Univ Florence, Dept Pharmaceut Sci, I-50019 Sesto Fiorentino, FI, Italy

[5] Univ Florence, Dept Neurol Sci, I-50134 Florence, Italy

[6] Univ Florence, Azienda Osped Careggi, I-50134 Florence, Italy

来源：

BIOPOLYMERS | 2010年 / 94卷 / 06期

关键词：

beta-turn structure; autoantibody; multiple sclerosis; glycopeptides; glycoamino acids; ASPARAGINE-LINKED OLIGOSACCHARIDES; DESIGNED GLYCOPEPTIDES; SYNTHETIC PROBES; BUILDING-BLOCKS; SIDE-CHAIN; MULTIPLE; AUTOANTIBODIES; GLYCOSYLATION; BIOMARKERS; PROTEIN;

D O I：

10.1002/bip.21456

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the design, synthesis, and immunological evaluation of glycoppeptide analogues of the previously described antigenic probe CSF114(Glo), with the aim of understanding the importance of N-glycosylation on Asn residue in multiple sclerosis antibody recognition The glucopeptide, characterized by a beta-turn conformation which is fundamental for a correct 1 presentation of the epitope, has been modified by introducing various natural glycoamino acids in position 7 The new glycopeptides were evaluated by measuring the IgG and IgM antibody titer in multiple sclerosis patients' and normal blood donors' sera Moreover, we achieved the efficient synthetic strategy of new Asn derivative bearing N-acetylneuraminic acid (Neu5Ac), linked by an glycosidic bond, on the side chain of the An reveille orthogonally protected for Fmoc/tBu SPPS 0 2010 Wiley Periodicals, Inc Biopolymers (Pept Sci) 94 791-799, 2010 Keywords beta-turn structu e autoantibody multiple sclerosis glycopeptides glycoamino acids

引用

页码：791 / 799

页数：9

共 31 条

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