Tailorable polyelectrolyte protein complex based on poly(aspartic acid) and bovine serum albumin

被引:10
作者
Nita, Loredana E. [1 ]
Chiriac, Aurica P. [1 ]
Stoleru, Elena [1 ]
Diaconu, Alina [1 ]
Tudorachi, Nita [1 ]
机构
[1] Petru Poni Inst Macromol Chem, Iasi, Romania
关键词
Polyelectrolyte-protein complex; bovine serum albumin; poly(aspartic acid);
D O I
10.1080/15685551.2016.1187436
中图分类号
O63 [高分子化学(高聚物)];
学科分类号
070305 ; 080501 ; 081704 ;
摘要
Protein-polyelectrolyte complexes (PPC) are playing an important role in a variety of chemical and biological processes, such as protein separation, enzyme stabilization, and polymer drug delivery. The present investigation is focused on evaluation of the PPC formation between a synthetic polypeptide (poly(aspartic acid) - PAS) and a natural protein (bovine serum albumin - BSA). The PPC obtained from PAS and BSA in different ratio was investigated by corroboration of various techniques of characterization as: spectroscopy, microscopy, thermogravimetric analysis, dynamic light scattering (DLS), and zeta potential determination, measurements which were performed in static and/or dynamic conditions. The static contact angle of the sample films was also determined in order to evaluate the changes brought upon surface free energy of the prepared PPCs in interdependence with the complexes composition. The critical conditions for complex formation in BSA-polyelectrolyte systems were underlined. The phenomenon was better evidenced in case of a ratio of 1/1 wt between the two polymers, both in static and/or dynamic conditions.
引用
收藏
页码:596 / 606
页数:11
相关论文
共 28 条
[1]  
Albu R.M., 2011, J BIOMATERIALS NANOB, V2, P114
[2]  
[Anonymous], 2013, Surface Science Techniques, DOI DOI 10.1007/978-3-642-34243-1_1
[3]  
[Anonymous], 1995, All about Albumin: Biochemistry, Genetics, and Medical Applications
[4]  
Chiriac AP, 2010, POLIMERY-W, V55, P641
[5]   Contribution to polymer nanoparticles analysis by laser light scattering [J].
Chiriac, Aurica P. ;
Nita, Loredana E. ;
Neamtu, Iordana ;
Bercea, Maria .
POLYMER TESTING, 2009, 28 (08) :886-890
[6]   Polyelectrolyte-protein complexes [J].
Cooper, CL ;
Dubin, PL ;
Kayitmazer, AB ;
Turksen, S .
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE, 2005, 10 (1-2) :52-78
[7]   Synthesis and Characterization of Bovine Serum Albumin-Conjugated Copper Sulfide Nanocomposites [J].
Huang, Peng ;
Li, Zhiming ;
Hu, Hengyao ;
Cui, Daxiang .
JOURNAL OF NANOMATERIALS, 2010, 2010
[8]   Intermolecular interactions between bovine serum albumin and certain water-soluble polymers at various temperatures [J].
Kavlak, S ;
Güner, A .
JOURNAL OF APPLIED POLYMER SCIENCE, 2006, 100 (02) :1554-1560
[9]   Surface characterization of biopolyurethanes based on cellulose derivatives [J].
Macocinschi, Doina ;
Filip, Daniela ;
Butnaru, Maria ;
Dimitriu, Cristina Daniela .
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2009, 20 (03) :775-783
[10]   Study of Bovine Serum Albumin Solubility in Aqueous Solutions by Intrinsic Viscosity Measurements [J].
Masuelli, Martin Alberto .
ADVANCES IN PHYSICAL CHEMISTRY, 2013,