The relationship between LDL oxidation and macrophage myeloperoxidase activity

被引：0

作者：

Wu, JZ ^{[1
]}

Liu, YH ^{[1
]}

Li, XM ^{[1
]}

Chen, LD ^{[1
]}

Xia, LJ ^{[1
]}

Hong, JL ^{[1
]}

机构：

[1] Wuhan Univ, Dept Biochem, Coll Med, Wuhan 430071, Peoples R China

来源：

CHINESE MEDICAL JOURNAL | 2003年 / 116卷 / 05期

关键词：

low density lipoprotein; oxidation; myeloperoxidase;

D O I：

暂无

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective To explore low density lipoprotein ( LDL) oxidation by macrophage myeloperoxidase (MPO) at molecular level. Methods Using a mouse macrophage model, we examined the relationship between LDL oxidation and macrophage MPO by measuring macrophage MPO activity, LDL oxidation products, MPO gene expression and cellular orientation of LDL oxidation. Results MPO gene expression increased to its maximum gradually when the concentration of LDL was increased, and then maintained at that level. NaN3 inhibied the elevation of MPO activity and LDL oxidation, which was LDL concentration-dependent. After the composition of macrophage membrane was roughly analyzed, it was determined that the contents of MPO and LDL in 5% sucrose were 7.667 and 21 times higher than those in 10% sucrose, respectively. Conclusion LDL is attached to the "microdomain" of the macrophage membrane in which LDL is oxidized by MPO.

引用

页码：791 / 793

页数：3

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