共 37 条
Optimization and characterization of a homogeneous carboxylic surface functionalization for silicon-based biosensing
被引:20
作者:
Chiado, Alessandro
[1
]
Palmara, Gianluca
[1
]
Ricciardi, Serena
[1
]
Frascella, Francesca
[1
]
Castellino, Micaela
[2
]
Tortello, Mauro
[1
]
Ricciardi, Carlo
[1
]
Rivolo, Paola
[1
]
机构:
[1] Politecn Torino, Dept Appl Sci & Technol, Cso Duca Abruzzi 24, I-10129 Turin, Italy
[2] Ist Italiano Tecnol, Ctr Space Human Robot, Cso Trento 21, I-10129 Turin, Italy
关键词:
Succinic anhydride;
Biosensing;
Carboxylic functionalization;
Immunoassay;
AFM;
XPS;
Protein G;
Oriented immobilization;
Signal to noise;
EDC/NHS;
SELF-ASSEMBLED MONOLAYERS;
ANTIBODY IMMOBILIZATION;
CONDUCTING POLYMER;
GLUTARALDEHYDE;
FABRICATION;
FILMS;
AMINOSILANE;
LIMITATIONS;
COMPOSITES;
OXIDASE;
D O I:
10.1016/j.colsurfb.2016.03.048
中图分类号:
Q6 [生物物理学];
学科分类号:
071011 ;
摘要:
A well-organized immobilization of bio-receptors is a crucial goal in biosensing, especially to achieve high reproducibility, sensitivity and specificity. These requirements are usually attained with a controlled chemical/biochemical functionalization that creates a stable layer on a sensor surface. In this work, a chemical modification protocol for silicon-based surfaces to be applied in biosensing devices is presented. An anhydrous silanization step through 3-aminopropylsilane (APTES), followed by a further derivatization with succinic anhydride (SA), is optimized to generate an ordered flat layer of carboxylic groups. The properties of APTES/SA modified surface were compared with a functionalization in which glutaraldehyde (GA) is used as crosslinker instead of SA, in order to have a comparison with an established and largely applied procedure. Moreover, a functionalization based on the controlled deposition of a plasma polymerized acrylic acid (PPAA) thin film was used as a reference for carboxylic reactivity. Advantages and drawbacks of the considered methods are highlighted, through physico-chemical characterizations (OCA, XPS, and AFM) and by means of a functional Protein G/Antibody immunoassay. These analyses reveal that the most homogeneous, reproducible and active surface is achieved by using the optimized APTES/SA coupling. (C) 2016 Elsevier B.V. All rights reserved.
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页码:252 / 259
页数:8
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