Optimization and characterization of a homogeneous carboxylic surface functionalization for silicon-based biosensing

被引:20
作者
Chiado, Alessandro [1 ]
Palmara, Gianluca [1 ]
Ricciardi, Serena [1 ]
Frascella, Francesca [1 ]
Castellino, Micaela [2 ]
Tortello, Mauro [1 ]
Ricciardi, Carlo [1 ]
Rivolo, Paola [1 ]
机构
[1] Politecn Torino, Dept Appl Sci & Technol, Cso Duca Abruzzi 24, I-10129 Turin, Italy
[2] Ist Italiano Tecnol, Ctr Space Human Robot, Cso Trento 21, I-10129 Turin, Italy
关键词
Succinic anhydride; Biosensing; Carboxylic functionalization; Immunoassay; AFM; XPS; Protein G; Oriented immobilization; Signal to noise; EDC/NHS; SELF-ASSEMBLED MONOLAYERS; ANTIBODY IMMOBILIZATION; CONDUCTING POLYMER; GLUTARALDEHYDE; FABRICATION; FILMS; AMINOSILANE; LIMITATIONS; COMPOSITES; OXIDASE;
D O I
10.1016/j.colsurfb.2016.03.048
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
A well-organized immobilization of bio-receptors is a crucial goal in biosensing, especially to achieve high reproducibility, sensitivity and specificity. These requirements are usually attained with a controlled chemical/biochemical functionalization that creates a stable layer on a sensor surface. In this work, a chemical modification protocol for silicon-based surfaces to be applied in biosensing devices is presented. An anhydrous silanization step through 3-aminopropylsilane (APTES), followed by a further derivatization with succinic anhydride (SA), is optimized to generate an ordered flat layer of carboxylic groups. The properties of APTES/SA modified surface were compared with a functionalization in which glutaraldehyde (GA) is used as crosslinker instead of SA, in order to have a comparison with an established and largely applied procedure. Moreover, a functionalization based on the controlled deposition of a plasma polymerized acrylic acid (PPAA) thin film was used as a reference for carboxylic reactivity. Advantages and drawbacks of the considered methods are highlighted, through physico-chemical characterizations (OCA, XPS, and AFM) and by means of a functional Protein G/Antibody immunoassay. These analyses reveal that the most homogeneous, reproducible and active surface is achieved by using the optimized APTES/SA coupling. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:252 / 259
页数:8
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