Effects of dietary lysine levels on apparent nutrient digestibility and cationic amino acid transporter mRNA abundance in the small intestine of finishing pigs, Sus scrofa

被引:25
作者
Wang, Xiu-Qi [1 ,2 ]
Zeng, Pei-Ling [1 ,2 ]
Feng, You [1 ,2 ]
Zhang, Chang-Ming [1 ]
Yang, Jing-Pei [1 ]
Shu, Gang [1 ,2 ]
Jiang, Qing-Yan [1 ,2 ]
机构
[1] S China Agr Univ, Dept Anim Sci, Guangzhou 510642, Guangdong, Peoples R China
[2] Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Guangzhou, Guangdong, Peoples R China
基金
中国国家自然科学基金;
关键词
apparent nutrient digestibility; cationic amino acid transporter; finishing pig; lysine; CARCASS CHARACTERISTICS; GROWTH-PERFORMANCE; MOLECULAR-CLONING; PORCINE SOMATOTROPIN; TISSUE DISTRIBUTION; IGF-I; EXPRESSION; PROTEIN; PLASMA; RESTRICTION;
D O I
10.1111/j.1740-0929.2011.00941.x
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
One hundred and twenty pigs were used to evaluate the effects of different dietary lysine levels on the growth performance, apparent nutrient digestibility, and abundance of cationic amino acid transporter messenger RNA (mRNA) in the small intestine of finishing pigs. Pigs received a low lysine diet (LL, 0.60% lysine), moderate lysine diet (ML, 0.80% lysine) or a high lysine diet (HL, 1.00% lysine) for 28 days. A digestion test was carried out during the third week. Although the apparent nutrient digestibility in pigs fed experimental diets were different (P < 0.05) and the highest when pigs were fed ML diet, diets did not change the growth performance. In the duodenum, mRNA abundance of PepT-1, as detected by real-time RT-PCR, was reduced in the LL diet (P < 0.05). A greater abundance of b0,+AT and PepT-1 mRNA was associated with the ML diet (P < 0.05) in the jejunum and ileum, respectively. In the ileum, the HL diet had a lower abundance of CAT-1 mRNA compared with other diets. These results showed that the finishing pigs would gain better nutrient digestibility when the dietary lysine content was 0.80%, and dietary lysine levels influenced the expression of cationic amino acid transporter mRNA in the small intestine of finishing pigs.
引用
收藏
页码:148 / 155
页数:8
相关论文
共 42 条
  • [1] [Anonymous], SAS US GUID VERS 8 2
  • [2] [Anonymous], 2002, Official Method of Analysis
  • [3] [Anonymous], 1998, NUTR REQ SWIN
  • [4] Apple JK, 2004, J ANIM SCI, V82, P3277
  • [5] Amino acid transport across mammalian intestinal and renal epithelia
    Broer, Stefan
    [J]. PHYSIOLOGICAL REVIEWS, 2008, 88 (01) : 249 - 286
  • [6] Cameron ND, 2003, J ANIM SCI, V81, P91
  • [7] Molecular mechanisms involved in the adaptation to amino acid limitation in mammals
    Chaveroux, Cedric
    Lambert-Langlais, Sarah
    Cherasse, Yoan
    Averous, Julien
    Parry, Laurent
    Carraro, Valerie
    Jousse, Celine
    Maurin, Anne-Catherine
    Bruhat, Alain
    Fafournoux, Pierre
    [J]. BIOCHIMIE, 2010, 92 (07) : 736 - 745
  • [8] Molecular cloning and functional expression of a chicken intestinal peptide transporter (cPepT1) in Xenopus oocytes and Chinese hamster ovary cells
    Chen, H
    Pan, YX
    Wong, EA
    Bloomquist, JR
    Webb, KE
    [J]. JOURNAL OF NUTRITION, 2002, 132 (03) : 387 - 393
  • [9] Effects of L-carnitine with different lysine levels on growth and nutrient digestibility in pigs weaned at 21 days of age
    Cho, WT
    Kim, JH
    Han, IK
    Han, YK
    Heo, KN
    Odle, J
    [J]. ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES, 1999, 12 (05): : 799 - 805
  • [10] Structure and function of cationic amino acid transporters (CATs)
    Closs, E. I.
    Boissel, J.-P.
    Habermeier, A.
    Rotmann, A.
    [J]. JOURNAL OF MEMBRANE BIOLOGY, 2006, 213 (02) : 67 - 77