The TRIM-NHL protein NHL-2 is a co-factor in the nuclear and somatic RNAi pathways in C. elegans

被引：11

作者：

Davis, Gregory M. ^{[1
,2
,3
]}

Tu, Shikui ^{[4
]}

Anderson, Joshua W. T. ^{[1
,2
]}

Colson, Rhys N. ^{[1
,2
]}

Gunzburg, Menachem J. ^{[1
,2
,7
]}

Francisco, Michelle A. ^{[5
]}

Ray, Debashish ^{[5
]}

Shrubsole, Sean P. ^{[1
,2
]}

Sobotka, Julia A. ^{[5
]}

Seroussi, Uri ^{[5
]}

Lao, Robert X. ^{[5
]}

Maity, Tuhin ^{[5
]}

Wu, Monica Z. ^{[5
]}

McJunkin, Katherine ^{[6
]}

Morris, Quaid D. ^{[5
]}

Hughes, Timothy R. ^{[5
]}

Wilce, Jacqueline A. ^{[1
,2
]}

Claycomb, Julie M. ^{[5
]}

Weng, Zhiping ^{[4
]}

Boag, Peter R. ^{[1
,2
]}

机构：

[1] Monash Univ, Monash Biomed Discovery Inst, Dev & Stem Cells Program, Melbourne, Vic, Australia

[2] Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic, Australia

[3] Federat Univ, Sch Hlth & Life Sci, Victoria, Vic, Australia

[4] Univ Massachusetts, Med Sch, Program Bioinformat & Integrat Biol, Worcester, MA 01605 USA

[5] Univ Toronto, Dept Mol Genet, Toronto, ON, Canada

[6] NIDDK, Lab Cellular & Dev Biol, NIH, Bethesda, MD USA

[7] Monash Univ, Monash Inst Pharmaceut Sci, Melbourne, Vic, Australia

来源：

ELIFE | 2018年 / 7卷

基金：

美国国家卫生研究院; 英国医学研究理事会; 加拿大健康研究院;

关键词：

PHYSIOLOGICAL GERMLINE APOPTOSIS; DROSOPHILA BRAIN-TUMOR; MESSENGER-RNA; GENE-EXPRESSION; FUNCTIONAL-CHARACTERIZATION; EPIGENETIC MEMORY; CAENORHABDITIS; PIRNAS; CSR-1; INTERFERENCE;

D O I：

10.7554/eLife.35478

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Proper regulation of germline gene expression is essential for fertility and maintaining species integrity. In the C. elegans germline, a diverse repertoire of regulatory pathways promote the expression of endogenous germline genes and limit the expression of deleterious transcripts to maintain genome homeostasis. Here we show that the conserved TRIM-NHL protein, NHL-2, plays an essential role in the C. elegans germline, modulating germline chromatin and meiotic chromosome organization. We uncover a role for NHL-2 as a co-factor in both positively (CSR-1) and negatively (HRDE-1) acting germline 22G-small RNA pathways and the somatic nuclear RNAi pathway. Furthermore, we demonstrate that NHL-2 is a bona fide RNA binding protein and, along with RNA-seq data point to a small RNA independent role for NHL-2 in regulating transcripts at the level of RNA stability. Collectively, our data implicate NHL-2 as an essential hub of gene regulatory activity in both the germline and soma.

引用

页数：29

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