Rainbow trout Oncorhynchus mykiss ladderlectin, but not intelectin, binds viral hemorrhagic septicemia virus IVb

被引：9

作者：

Reid, A. ^{[1
]}

Young, K. M. ^{[1
]}

Lumsden, J. S. ^{[1
]}

机构：

[1] Univ Guelph, Ontario Vet Coll, Dept Pathobiol, Fish Pathol Lab, Guelph, ON N1G 2W1, Canada

来源：

DISEASES OF AQUATIC ORGANISMS | 2011年 / 95卷 / 02期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Oncorhychus mykiss; Pattern recognition receptor; Viral hemorrhagic septicemia virus; Innate immunity; Plasma proteins; Binding; Ladderlectin; Intelectin; IMMUNE-RESPONSES; GLYCOPROTEIN-G; TISSUE LOCALIZATION; INFLUENZA-VIRUS; HOST-DEFENSE; LECTIN; FISH; RHABDOVIRUS; INFECTION; IDENTIFICATION;

D O I：

10.3354/dao02358

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

The innate immune system of fish is critical for rapid detection and immediate response to infection, as well as to orchestrate the adaptive branch of the immune system. Rainbow trout Oncorhynchus mykiss ladderlectin and intelectin are plasma pattern recognition receptors (PRR) for bacterial and fungal pathogens of rainbow trout, but their role as PRRs for virus is unknown. Viral hemorrhagic septicemia virus (VHSV) IVb is a recently described fish pathogen in the Great Lakes, and rainbow trout can be experimentally infected. Using an indirect enzyme-linked immuno sorbent assay, rainbow trout plasma ladderlectin significantly (p < 0.05) bound purified VHSV while intelectin did not. In addition, plasma ladderlectin but not intelectin was eluted from a VHSV-conjugated Toyopearl column using EDTA. Protein identification was confirmed with polyclonal antiserum used with slot immunoblot, 1-dimensional sodium dodecyl sulphate polyacrylamide electrophoresis, and Western immunoblot.

引用

页码：137 / 143

页数：7

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