Xenogeneic immunosuppression of human umbilical cord mesenchymal stem cells in a major histocompatibility complex-mismatched allogeneic acute graft-versus-host disease murine model

被引：19
作者：
Guo, Juan [1 ]
Yang, Jie [2 ]
Cao, Guofan [2 ,3 ]
Fan, Huahua [2 ]
Guo, Chenzhi [2 ,3 ]
Ma, Yue-e [2 ]
Qian, Yanxiang [4 ]
Chen, Liang [4 ]
Li, Xiao [1 ]
Chang, Chunkang [1 ]
机构：
[1] Sixth Peoples Hosp, Dept Hematol, Shanghai 200233, Peoples R China
[2] Shanghai Blood Ctr, Blood Engn Lab, Shanghai 200051, Peoples R China
[3] E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China
[4] Cord Blood Stem Cell Bank Shanghai, Shanghai, Peoples R China
来源：
EUROPEAN JOURNAL OF HAEMATOLOGY | 2011年 / 87卷 / 03期
关键词：
human umbilical cord mesenchymal stem cells; arginase; 1; IFN-gamma; indoleamine 2,3-dioxygenase; immunosuppression; acute graft-versus-host disease; STROMAL CELLS; SUPPRESSOR-CELLS; T-CELLS; INDOLEAMINE 2,3-DIOXYGENASE; MEDIATED IMMUNOSUPPRESSION; STEROID-RESISTANT; INTERFERON-GAMMA; NITRIC-OXIDE; RESPONSES; THERAPY;
D O I：
10.1111/j.1600-0609.2011.01635.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Mesenchymal stem cells (MSCs) hold great promise for treating immune disorders owing to their immunoregulatory capacity, but the mechanism remains controversial. As we show here, the mechanism of human umbilical cord mesenchymal stem cell (HUCMSC)-mediated immunosuppression involves TGF-beta and indoleamine 2,3-dioxygenase (IDO). In this study, we investigated the influence of xenogeneic HUCMSCs on acute graft-versus-host disease (aGVHD) in murine allogeneic bone marrow transplantation (BMT). In the HUCMSC-treated group, lethally irradiated DBA/2(H-2Kd) mice were adoptively transferred with expanded HUCMSCs, bone marrow (BM), and splenocytes (SCs) from C57BL/6 (H-2Kb) mice. Recipients in the control group were transferred only BM and SCs. The two groups were compared in survival, weight, histopathologic specimens, and aGVHD scoring. In the HUCMSC-treated group, 60% of the mice survived past day 30 after BMT, but in the control group, all mice died within 18 d. The mice treated with HUCMSCs exhibited light symptoms of aGVHD after day 30. The results suggest that xenogeneic HUCMSCs could alleviate aGVHD symptoms and prolong survival after allogeneic BMT. Our study suggests that in vitro expanded HUCMSCs might be used to inhibit severe aGVHD effectively in allogeneic hematopoietic cell transplantation clinically.
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页码：235 / 243
页数：9
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