Identification and chromosome mapping of repetitive elements in the Astyanax scabripinnis (Teleostei: Characidae) species complex

被引：23

作者：

Barbosa, Patricia ^{[1
,2
]}

de Oliveira, Luiz Antonio ^{[1
]}

Pucci, Marcela Baer ^{[1
,2
]}

Santos, Mateus Henrique ^{[1
]}

Moreira-Filho, Orlando ^{[1
,2
]}

Vicari, Marcelo Ricardo ^{[1
]}

Nogaroto, Viviane ^{[1
]}

de Almeida, Mara Cristina ^{[1
]}

Artoni, Roberto Ferreira ^{[1
,2
]}

机构：

[1] Univ Estadual Ponta Grossa, Dept Biol Estrutural Mol & Genet, Programa Pos Grad Biol Evolut, BR-84030900 Ponta Grossa, PR, Brazil

[2] Univ Fed Sao Carlos, Dept Genet & Evolucao, Programa Pos Grad Genet Evolut & Biol Mol, BR-13565905 Sao Carlos, SP, Brazil

来源：

GENETICA | 2015年 / 143卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

rDNA; Retrotransposon; Satellite DNA; B chromosome; TRANSPOSABLE ELEMENTS; B-CHROMOSOMES; CYTOGENETIC ANALYSIS; BOCKMANNI TELEOSTEI; SATELLITE DNA; CHARACIFORMES; SEQUENCES; PISCES; GENOME; COLOCALIZATION;

D O I：

10.1007/s10709-014-9813-2

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Most part of the eukaryotic genome is composed of repeated sequences or multiple copies of DNA, which were considered as "junk DNA'', and may be associated to the heterochromatin. In this study, three populations of Astyanax aff. scabripinnis from Brazilian rivers of Guaratingueta and Pindamonhangaba (Sao Paulo) and a population from Maringa (Parana) were analyzed concerning the localization of the nucleolar organizer regions (Ag-NORs), the As51 satellite DNA, the 18S ribosomal DNA (rDNA), and the 5S rDNA. Repeated sequences were also isolated and identified by the C(o)t - 1 method, which indicated similarity (90 %) with the LINE UnaL2 retrotransposon. The fluorescence in situ hybridization (FISH) showed the retrotransposon dispersed and more concentrated markers in centromeric and telomeric chromosomal regions. These sequences were co-localized and interspaced with 18S and 5S rDNA and As51, confirmed by fiber-FISH essay. The B chromosome found in these populations pointed to a conspicuous hybridization with LINE probe, which is also co-located in As51 sequences. The NORs were active at unique sites of a homologous pair in the three populations. There were no evidences that transposable elements and repetitive DNA had influence in the transcriptional regulation of ribosomal genes in our analyses.

引用

页码：55 / 62

页数：8

共 39 条

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