Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 in rat calvarial defects

被引:41
作者
Kim, Jin-Woo [1 ]
Choi, Kyung-Hee [2 ]
Yun, Jeong-Ho [1 ]
Jung, Ui-Won [1 ]
Kim, Chang-Sung [1 ]
Choi, Seong-Ho [1 ]
Cho, Kyoo-Sung [1 ]
机构
[1] Yonsei Univ, Coll Dent, Dept Periodontol, Res Inst Periodontal Regenerat, Seoul 120749, South Korea
[2] Cowellmedi Co, Inst Dev Res, Pusan, South Korea
来源
ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTOLOGY | 2011年 / 112卷 / 03期
关键词
BETA-TRICALCIUM PHOSPHATE; SINUS AUGMENTATION; REGENERATION; CERAMICS; AUTOGRAFT; CARRIERS; RHBMP-2; REPAIR; HYDROXYAPATITE; SYSTEM;
D O I
10.1016/j.tripleo.2010.10.025
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The objective of this study was to evaluate bone formation in rat calvarial defects after surgical implantation of block or particulated biphasic calcium phosphate (BCP) lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 (ErhBMP-2). Critical-size calvarial osteotomy defects were created in 5 groups of Sprague-Dawley rats. Each group received one of the following: 1) sham surgery control; 2) biphasic calcium phosphate particles (CPP); 3) biphasic calcium phosphate block (CPB); 4) ErhBMP-2-coated CPP; or 5) ErhBMP-2-coated CPB. ErhBMP was coated on BCP by a stepwise lyophilizing protocol. The new bone formation was significantly greater in ErhBMP-2-treated groups compared with the untreated group. In particular, the ErhBMP-2/CPB group showed stability of augmented areas during the period of healing, due to relevant space-providing capacity. Thus, it can be concluded that CPP and CPB lyophilized with ErhBMP-2 enhance the formation of new bone, and CPB appears to be a suitable carrier for ErhBMP-2 in which a 3-dimensional structural integrity is an important consideration factor. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011; 112:298-306)
引用
收藏
页码:298 / 306
页数:9
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