RETRACTED: The Expression of the Long Noncoding RNA AFAP1-AS1 in Laryngeal Carcinoma Affects the Proliferation, Invasion, Migration, and Apoptosis of TU212 Cell Line (Retracted Article)

被引：2

作者：

Chen, Xin ^{[1
]}

Hu, Ziwei ^{[2
]}

Bing, Liao ^{[3
]}

Zhu, Xinhua ^{[3
]}

Liu, Ke ^{[3
]}

Liu, Yuehui ^{[3
]}

Liu, Jianguo ^{[3
]}

机构：

[1] Youxian Peoples Hosp Hunan Prov, Otolaryngol Dept, Youxian, Peoples R China

[2] Jinan Univ, Dept Otolaryngol & Head & Neck Surg, Guangzhou Red Cross Hosp, Guangzhou, Guangdong, Peoples R China

[3] Nanchang Univ, Otolaryngol Head & Neck Surg, Affiliated Hosp 2, Nanchang, Jiangxi, Peoples R China

来源：

BIOMED RESEARCH INTERNATIONAL | 2022年 / 2022卷

关键词：

LNCRNA; PROGRESSION;

D O I：

10.1155/2022/2337447

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background. lncRNA AFAP1-AS1 has been linked to the pathogenesis of a wide range of tumors. Nevertheless, whether it plays a role in laryngeal carcinoma (LC) remains unclear. Methods. Twenty-nine pairs of LC and related normal tissues were collected for the detection of lncRNA AFAP1-AS1 using qRT-PCR. Correlation of lncRNA AFAP1-AS1 level and clinicopathological characters was assessed by the chi-square test. Impacts of lncRNA AFAP1-AS1 silencing on LC phenotypes were tested in vitro via CCK-8, clone formation, EdU staining, wound healing, flow cytometry, and Transwell assay. Results. Herein, a remarkable elevation of lncRNA AFAP1-AS1 was observed in LC patients. And higher lncRNA AFAP1-AS1 level was correlated to worse clinical pathological characteristics. Moreover, lncRNA AFAP1-AS1 silencing was revealed to repress TU212 malignant phenotypes. Conclusion. Our data suggested that lncRNA AFAP1-AS1 acts as an oncogene of LC in vitro.

引用

页数：8

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