Effect of Rta protein of Epstein-Barr virus on the cell cycle in HeLa cells

被引：4

作者：

Guo, Qingwei ^{[2
]}

Sun, Xiaobai ^{[3
]}

Yuan, Changjin ^{[1
]}

Zhou, Huijuan ^{[1
]}

Li, Yanmei ^{[1
]}

Jie, Gao ^{[1
]}

Jiang, Guosheng ^{[1
]}

机构：

[1] Shandong Acad Med Sci, Shandong Key Lab Rare & Uncommon Dis, Inst Basic Med, Jinan 250062, Peoples R China

[2] Shandong Univ, Qilu Childrens Hosp, Jinan 250022, Peoples R China

[3] Adicona Clin Labs, Jinan 250031, Peoples R China

来源：

ACTA VIROLOGICA | 2011年 / 55卷 / 04期

关键词：

Epstein-Barr virus; Rta; Rb; p53; E2F-1; cell cycle; HeLa cells; TRANSCRIPTION FACTOR; LYTIC REPLICATION; B-CELLS; S-PHASE; BRLF1; ACTIVATION; INFECTION; PROGRESSION; BINDING; GENES;

D O I：

10.4149/av_2011_04_311

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Epstein-Barr virus (EBV) replication and transcription activator (Rta) is an immediate-early transcription factor that mediates the switch from latent to lytic infection. DNA viruses often modulate the function of critical cell cycle proteins to maximize the efficiency of virus replication. Here we have examined the effect of Rta on cell cycle progression. Cell cycle analysis revealed that Rta induced HeLa cells in G0/G1-phase to reenter the S-phase. Analysis of the expression pattern of a key set of cell cycle regulators revealed that expression of Rta inhibited the expression of Rb and p53 and induced the expression of E2F1. These findings suggest that Rta plays an active role in redirecting HeLa cell physiology through an Rta-mediated cell cycle transformation.

引用

页码：311 / 316

页数：6

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