Mechanisms involved in quinolone resistance in Mycoplasma mycoides subsp capri

被引：22
作者：
Antunes, Nuno T. [1 ]
Assuncao, Patricia [1 ]
Poveda, Jose B. [1 ]
Tavio, Maria M. [1 ,2 ]
机构：
[1] Univ Las Palmas Gran Canaria, IUSA, Unidad Epidemiol & Med Prevent, Arucas 35413, Spain
[2] Univ Las Palmas Gran Canaria, Fac Ciencias Salud, Dept Ciencias Clin, Microbiol, Las Palmas Gran Canaria 35016, Spain
来源：
VETERINARY JOURNAL | 2015年 / 204卷 / 03期
关键词：
Mycoplasma mycoides subsp capri; Antibiotic resistance; Fluoroquinolones; Quinolone resistance determining regions; Efflux pumps; IN-VITRO SUSCEPTIBILITIES; EFFLUX-MEDIATED RESISTANCE; DNA GYRASE; FLUOROQUINOLONE RESISTANCE; TOPOISOMERASE-IV; CONTAGIOUS AGALACTIA; ETHIDIUM-BROMIDE; HOMINIS; MUTATIONS; MUTANTS;
D O I：
10.1016/j.tvjl.2015.04.018
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Mycoplasma mycoides subsp. capri is a causative agent of contagious agalactia in goats. In this study, M. mycoides subsp. capri mutants were selected for resistance to fluoroquinolones (norfloxacin, enrofloxacin and ciprofloxacin) by serial passes in broth with increasing concentrations of antibiotic. Mutations conferring cross-resistance to the three fluoroquinolones were found in the quinolone resistance determining regions of the four genes encoding DNA gyrase and topoisomerase IV. Different mutations in the DNA gyrase GyrA subunit suggest a different mechanism of inhibition between norfloxacin and the other tested fluoroquinolones. The presence of an adenosine triphosphate-dependent efflux system was suggested through the use of the inhibitor orthovanadate. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码：327 / 332
页数：6
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