Analysis of the genetic architecture of maize kernel size traits by combined linkage and association mapping

被引：88

作者：

Liu, Min ^{[1
]}

Tan, Xiaolong ^{[1
]}

Yang, Yan ^{[1
]}

Liu, Peng ^{[1
]}

Zhang, Xiaoxiang ^{[1
]}

Zhang, Yinchao ^{[1
]}

Wang, Lei ^{[1
]}

Hu, Yu ^{[1
]}

Ma, Langlang ^{[1
]}

Li, Zhaoling ^{[1
]}

Zhang, Yanling ^{[1
]}

Zou, Chaoying ^{[1
]}

Lin, Haijian ^{[1
]}

Gao, Shibin ^{[1
]}

Lee, Michael ^{[2
]}

Lubberstedt, Thomas ^{[2
]}

Pan, Guangtang ^{[1
]}

Shen, Yaou ^{[1
,3
]}

机构：

[1] Sichuan Agr Univ, Maize Res Inst, Key Lab Biol & Genet Improvement Maize Southwest, Chengdu, Peoples R China

[2] Iowa State Univ, Dept Agron, Ames, IA USA

[3] State Key Lab Crop Gene Explorat & Utilizat South, Chengdu, Peoples R China

来源：

PLANT BIOTECHNOLOGY JOURNAL | 2020年 / 18卷 / 01期

关键词：

maize; kernel size; genome-wide association study; QTL mapping; co-localization; functional genes; PENTATRICOPEPTIDE REPEAT PROTEIN; RICE GRAIN WIDTH; NAD2; INTRON; SEED DEVELOPMENT; ORGAN SIZE; PPR PROTEIN; MAJOR QTL; POPULATION-STRUCTURE; MICRORNA REGULATION; REGULATORY MODULE;

D O I：

10.1111/pbi.13188

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Kernel size-related traits are the most direct traits correlating with grain yield. The genetic basis of three kernel traits of maize, kernel length (KL), kernel width (KW) and kernel thickness (KT), was investigated in an association panel and a biparental population. A total of 21 single nucleotide polymorphisms (SNPs) were detected to be most significantly (P < 2.25 x 10(-6)) associated with these three traits in the association panel under four environments. Furthermore, 50 quantitative trait loci (QTL) controlling these traits were detected in seven environments in the intermated B73 x Mo17 (IBM) Syn10 doubled haploid (DH) population, of which eight were repetitively identified in at least three environments. Combining the two mapping populations revealed that 56 SNPs (P < 1 x 10(-3)) fell within 18 of the QTL confidence intervals. According to the top significant SNPs, stable-effect SNPs and the co-localized SNPs by association analysis and linkage mapping, a total of 73 candidate genes were identified, regulating seed development. Additionally, seven miRNAs were found to situate within the linkage disequilibrium (LD) regions of the co-localized SNPs, of which zma-miR164e was demonstrated to cleave the mRNAs of Arabidopsis CUC1, CUC2 and NAC6 in vitro. Overexpression of zma-miR164e resulted in the down-regulation of these genes above and the failure of seed formation in Arabidopsis pods, with the increased branch number. These findings provide insights into the mechanism of seed development and the improvement of molecular marker-assisted selection (MAS) for high-yield breeding in maize.

引用

页码：207 / 221

页数：15

共 106 条

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