A liver-specific isoform of the betaine/GABA transporter in the rat: cDNA sequence and organ distribution

被引：32

作者：

Burnham, CE ^{[1
]}

Buerk, B ^{[1
]}

Schmidt, C ^{[1
]}

Bucuvalas, JC ^{[1
]}

机构：

[1] CHILDRENS HOSP,MED CTR,DEPT PEDIAT GASTROENTEROL & NUTR,CINCINNATI,OH 45229

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 1996年 / 1284卷 / 01期

关键词：

betaine; osmolyte; membrane transporter;

D O I：

10.1016/0005-2736(96)00118-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the cloning of a 2.2 kb cDNA encoding a Na+- and Cl--dependent betaine/GABA (gamma-aminobutyric acid) transporter from rat liver poly(A(+)) RNA. 5'-RACE revealed an additional 355 bases 5' to the 2.2 kb cDNA sequence. Northern analysis demonstrated two (2.2 kb and 2.6 kb) mRNA isoforms in rat liver. Betaine transporter mRNA was also detected in the brain, spleen, lung, and kidney using the 2.2 kb cDNA clone as a probe. Only the 2.6 kb mRNA from the liver hybridized with the 5'-RACE product.

引用

页码：4 / 8

页数：5

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