Silencing of yellow head virus replication in penaeid shrimp cells by dsRNA

被引：135

作者：

Tirasophon, W ^{[1
]}

Roshorm, Y

Panyim, S

机构：

[1] Mahidol Univ, Inst Mol Biol & Genet, Salaya 73170, Nakornpathom, Thailand

[2] Mahidol Univ, Fac Sci, Dept Biochem, Bangkok 10400, Thailand

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 334卷 / 01期

关键词：

shrimp; Penaeus monodon; yellow head virus; RNAi; double-stranded RNA;

D O I：

10.1016/j.bbrc.2005.06.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

RNA interference (RNAi) has been shown to inhibit viral replication in some animals and plants. Whether the RNAi is functional in shrimp remains to be demonstrated. In vitro transcribed dsRNAs of YHV helicase, polymerase, protease, gp116, and gp64 were transfected into shrimp primary cell culture and found to inhibit YHV replication. dsRNA targeted to nonstructural genes (protease, polymerase, and helicase) effectively inhibited YHV replication. Those targeted structural genes (gp116 and gp64) were the least effective. These findings are the first evidence that RNAi-mediated gene silencing is operative in shrimp cells. This could be a powerful tool for studying gene function and to develop effective control of viral infection in shrimp. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：102 / 107

页数：6

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