Tear Analytical Model Based on Raman Microspectroscopy for Investigation of Infectious Diseases of the Ocular Surface

被引:37
作者
Kuo, Ming-Tse [1 ,3 ]
Lin, Chi-Chang [4 ]
Liu, Hsin-Yu [1 ]
Chang, Hsien-Chang [1 ,2 ]
机构
[1] Natl Cheng Kung Univ, Inst Biomed Engn, Tainan 70101, Taiwan
[2] Natl Cheng Kung Univ, Ctr Micro Nano Sci & Technol, Tainan 70101, Taiwan
[3] Chang Gung Univ, Dept Ophthalmol, Chang Gung Mem Hosp, Kaohsiung Med Ctr,Coll Med, Kaohsiung, Taiwan
[4] Tunghai Univ, Dept Chem & Mat Engn, Taichung 40704, Taiwan
关键词
HERPES-SIMPLEX-VIRUS; CORNEAL ULCERS; PSEUDOMONAS-AERUGINOSA; MICROBIAL KERATITIS; MYCOTIC KERATITIS; SPECTROSCOPY; FLUID; BACTERIAL; DIAGNOSIS; EFFICACY;
D O I
10.1167/iovs.10-7062
中图分类号
R77 [眼科学];
学科分类号
100212 ;
摘要
PURPOSE. To establish a tear analytical model by using Raman microspectroscopy to assess ocular surface diseases associated with infectious pathogens. METHODS. The authors applied confocal Raman microspectroscopy based on the drop-coating deposition method on Ti/Au-coated glass slides to obtain sample spectra from different types of tears, including simplified synthetic tears (SSTs), SSTs mixed with microbes, and human tears. Raman spectra were sampled by a line-mapping procedure and classified into three groups by three different zones in a dried teardrop. To determine the tear model with optimal discrimination, the spectra of the three zones were compared using spectral morphology and principal component analysis. Finally, the optimal tear model was verified by comparing the Raman spectra of human teardrops of patients with ulcerative keratitis and bacterial infection with those of patients without any identifiable infection. RESULTS. Nonhomogeneous intensities of Raman spectra collected by a line-mapping sampling procedure were found in different locations of a dried teardrop. This might have been caused by coffee-ring formation and ferny crystallization phenomena. The normalized spectra in the central zone have better discriminative potential than those in the other zones, ring zone, and transitional zone, tested by pure SSTs or SSTs with microbes. The tear model based on normalized Raman spectra in the central zone was discriminative for patients with ulcerative keratitis in the presence or absence of infection. CONCLUSIONS. Raman spectra in the central zone of a dried teardrop may serve as useful spectral fingerprints for investigating ocular surface diseases with or without infection. (Invest Ophthalmol Vis Sci. 2011;52:4942-4950) DOI:10.1167/iovs.10-7062
引用
收藏
页码:4942 / 4950
页数:9
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