Comparison between PCR and detection of antigen in sera for diagnosis of pulmonary aspergillosis

被引：70
作者：
Kawamura, S [1 ]
Maesaki, S [1 ]
Noda, T [1 ]
Hirakata, Y [1 ]
Tomono, K [1 ]
Tashiro, T [1 ]
Kohno, S [1 ]
机构：
[1] Nagasaki Univ, Sch Med, Dept Internal Med 2, Nagasaki 8528501, Japan
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 01期
关键词：
D O I：
10.1128/JCM.37.1.218-220.1999
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
We evaluated the usefulness of PCR and antigen detection for the diagnosis of pulmonary aspergillosis. Forty-four serum samples from patients with pulmonary aspergillosis (33 with pulmonary aspergilloma, 4 with allergic bronchopulmonary aspergillosis, 4 with invasive pulmonary aspergillosis, and 3 with aspergillus pyothorax) were used in this study. PCR detection of Aspergillus DNA in serum samples was successful in 39 patients. Galactomannan antigen was detected by sandwich enzyme-linked immunosorbent assay in 25 patients and by latex agglutination test in 13 patients. Detection of Aspergillus DNA in serum samples by nested PCR had the highest sensitivity of the three methods tested for the diagnosis of pulmonary aspergillosis.
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页码：218 / 220
页数：3
相关论文
共 11 条
  • [1] DETECTION OF ASPERGILLUS SPECIES DNA IN BRONCHOALVEOLAR LAVAGE SAMPLES BY COMPETITIVE PCR
    BRETAGNE, S
    COSTA, JM
    MARMORATKHUONG, A
    PORON, F
    CORDONNIER, C
    VIDAUD, M
    FLEURYFEITH, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (05) : 1164 - 1168
  • [2] PULMONARY ASPERGILLOMA - DIAGNOSTIC AND THERAPEUTIC CONSIDERATIONS
    GLIMP, RA
    BAYER, AS
    [J]. ARCHIVES OF INTERNAL MEDICINE, 1983, 143 (02) : 303 - 308
  • [3] AVOIDING FALSE POSITIVES WITH PCR
    KWOK, S
    HIGUCHI, R
    [J]. NATURE, 1989, 339 (6221) : 237 - 238
  • [4] LATGE JP, 1994, INFECT IMMUN, V62, P5424
  • [5] REPENTIGNY L, 1989, EUR J CLIN MICROBIOL, V8, P362
  • [6] Prospective sandwich enzyme-linked immunosorbent assay for serum galactomannan: Early predictive value and clinical use in invasive aspergillosis
    Rohrlich, P
    Sarfati, J
    Mariani, P
    Duval, M
    Carol, A
    SaintMartin, C
    Bingen, E
    Latge, JP
    Vilmer, E
    [J]. PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1996, 15 (03) : 232 - 237
  • [7] CLINICAL AND IMMUNOLOGICAL CRITERIA FOR DIAGNOSIS OF ALLERGIC BRONCHOPULMONARY ASPERGILLOSIS
    ROSENBERG, M
    PATTERSON, R
    MINTZER, R
    COOPER, BJ
    ROBERTS, M
    HARRIS, KE
    [J]. ANNALS OF INTERNAL MEDICINE, 1977, 86 (04) : 405 - 414
  • [8] A NEW SENSITIVE SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY TO DETECT GALACTOFURAN IN PATIENTS WITH INVASIVE ASPERGILLOSIS
    STYNEN, D
    GORIS, A
    SARFATI, J
    LATGE, JP
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (02) : 497 - 500
  • [9] Comparison of an enzyme immunoassay and latex agglutination test for detection of galactomannan in the diagnosis of invasive aspergillosis
    Sulahian, A
    Tabouret, M
    Ribaud, P
    Sarfati, J
    Gluckman, E
    Latge, JP
    Derouin, F
    [J]. EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1996, 15 (02) : 139 - 145
  • [10] THE DETECTION OF ASPERGILLUS SPP BY THE POLYMERASE CHAIN-REACTION AND ITS EVALUATION IN BRONCHOALVEOLAR LAVAGE FLUID
    TANG, CM
    HOLDEN, DW
    AUFAUVREBROWN, A
    COHEN, J
    [J]. AMERICAN REVIEW OF RESPIRATORY DISEASE, 1993, 148 (05): : 1313 - 1317
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