Comparison between PCR and detection of antigen in sera for diagnosis of pulmonary aspergillosis

被引：70

作者：

Kawamura, S ^{[1
]}

Maesaki, S ^{[1
]}

Noda, T ^{[1
]}

Hirakata, Y ^{[1
]}

Tomono, K ^{[1
]}

Tashiro, T ^{[1
]}

Kohno, S ^{[1
]}

机构：

[1] Nagasaki Univ, Sch Med, Dept Internal Med 2, Nagasaki 8528501, Japan

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1999年 / 37卷 / 01期

关键词：

D O I：

10.1128/JCM.37.1.218-220.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated the usefulness of PCR and antigen detection for the diagnosis of pulmonary aspergillosis. Forty-four serum samples from patients with pulmonary aspergillosis (33 with pulmonary aspergilloma, 4 with allergic bronchopulmonary aspergillosis, 4 with invasive pulmonary aspergillosis, and 3 with aspergillus pyothorax) were used in this study. PCR detection of Aspergillus DNA in serum samples was successful in 39 patients. Galactomannan antigen was detected by sandwich enzyme-linked immunosorbent assay in 25 patients and by latex agglutination test in 13 patients. Detection of Aspergillus DNA in serum samples by nested PCR had the highest sensitivity of the three methods tested for the diagnosis of pulmonary aspergillosis.

引用

页码：218 / 220

页数：3

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