Multicolor Super-resolution Fluorescence Microscopy with Blue and Carmine Small Photoblinking Polymer Dots

被引：72

作者：

Chen, Xuanze ^{[1
,2
]}

Liu, Zhihe ^{[3
,4
]}

Li, Rongqin ^{[1
,2
]}

Shan, Chunyan ^{[1
,2
]}

Zeng, Zhiping ^{[5
]}

Xue, Boxin ^{[1
,2
]}

Yuan, Weihong ^{[1
,2
]}

Mo, Chi ^{[1
,2
]}

Xi, Peng ^{[1
,2
]}

Wu, Changfeng ^{[3
]}

Sun, Yujie ^{[1
,2
]}

机构：

[1] Peking Univ, Sch Life Sci, State Key Lab Membrane Biol, Biodynam Opt Imaging Ctr BIOPIC, Beijing 100871, Peoples R China

[2] Peking Univ, Dept Biomed Engn, Coll Engn, Beijing 100871, Peoples R China

[3] Southern Univ Sci & Technol, Dept Biomed Engn, Shenzhen 510855, Guangdong, Peoples R China

[4] Jilin Univ, Coll Elect Sci & Engn, State Key Lab Integrated Optoelect, Changchun 130012, Jilin, Peoples R China

[5] Fuzhou Univ, Coll Phys & Informat Engn, Fuzhou 350116, Fujian, Peoples R China

来源：

ACS NANO | 2017年 / 11卷 / 08期

基金：

中国国家自然科学基金;

关键词：

multicolor super-resolution imaging; super-resolution optical fluctuation imaging; blue and carmine small polymer dots; photoblinking; bioimaging; QUANTUM DOTS; NANOPARTICLES; ACETYLATION; EMISSION; BIOLOGY; PROTEIN; DESIGN; NARROW; LIMIT;

D O I：

10.1021/acsnano.7b02893

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Advances in the development of small photoblinking semiconducting polymer dots (Pdots) have attracted great interest for use in super-resolution microscopy. However, multicolor super-resolution imaging using conventional small photoblinking Pdots remains a challenge due to their limited color choice, broad emission spectrum, and heavy spectrum crosstalk. Here, we introduce two types of small photoblinking Pdots with different colors and relatively narrow emission spectra: blue PFO Pdots and carmine PFTBTS Pdots for blinking-based statistical nanoscopy. Both of these probes feature ultrahigh single-particle brightness, very strong photostability, superior biocompatibility, and robust fluorescence fluctuation. In addition, these small photoblinking Pdots serve as excellent labels for dual-color super resolution optical fluctuation imaging (SOFT) of specific subcellular structures, indicating their promise for long-term multicolor SOFI nanoscopy with high spatiotemporal resolution.

引用

页码：8084 / 8091

页数：8

共 46 条

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