siRNAs and shRNAs: Skeleton keys to the human genome

被引：0

作者：

Paddison, PJ ^{[1
]}

Hannon, GJ ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 1172 USA

来源：

CURRENT OPINION IN MOLECULAR THERAPEUTICS | 2003年 / 5卷 / 03期

关键词：

functional genomics; gene silencing; human disease; mouse models; RNAi; shRNA; siRNA;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of RNA interference (RNAi) to evoke gene silencing in mammalian cell systems is rapidly becoming a standard laboratory technique. Recent experiments have also proven the utility of RNAi in animals. The next few years will bear witness to the first RNAi-based genome-wide screens in mammals, breaking the barrier to the use of mammalian cells as a genetic system. In this review, some Of the key features of RNAi in mammalian systems are discussed.

引用

页码：217 / 224

页数：8

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