siRNAs and shRNAs: Skeleton keys to the human genome

被引：0

作者：

Paddison, PJ ^{[1
]}

Hannon, GJ ^{[1
]}

机构：

[1] Cold Spring Harbor Lab, Watson Sch Biol Sci, Cold Spring Harbor, NY 1172 USA

来源：

CURRENT OPINION IN MOLECULAR THERAPEUTICS | 2003年 / 5卷 / 03期

关键词：

functional genomics; gene silencing; human disease; mouse models; RNAi; shRNA; siRNA;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of RNA interference (RNAi) to evoke gene silencing in mammalian cell systems is rapidly becoming a standard laboratory technique. Recent experiments have also proven the utility of RNAi in animals. The next few years will bear witness to the first RNAi-based genome-wide screens in mammals, breaking the barrier to the use of mammalian cells as a genetic system. In this review, some Of the key features of RNAi in mammalian systems are discussed.

引用

页码：217 / 224

页数：8

共 50 条

[1] Design and validation of siRNAs and shRNAs
Tilesi, Francesca
Fradiani, Piera
Socci, Valentina
Willems, Daniela
Ascenzioni, Fiorentina
CURRENT OPINION IN MOLECULAR THERAPEUTICS, 2009, 11 (02) : 156 - 164
[2] Functional and intracellular localization properties of U6 promoter-ex pressed siRNAs, shRNAs, and chimeric VA1 shRNAs in mammalian cells
Lee, Nan Sook
Kim, Daniel H.
Alluin, Jessica
Robins, Marjorie
Gu, Shuo
Li, Haitang
Kim, James
Salvaterra, Paul M.
Rossi, John J.
RNA, 2008, 14 (09) : 1823 - 1833
[3] piRNAs and siRNAs collaborate in Caenorhabditis elegans genome defense
Allison C Billi
Mallory A Freeberg
John K Kim
Genome Biology, 13
[4] piRNAs and siRNAs collaborate in Caenorhabditis elegans genome defense
Billi, Allison C.
Freeberg, Mallory A.
Kim, John K.
GENOME BIOLOGY, 2012, 13 (07):
[5] Suppression of hepatitis A virus genome translation and replication by siRNAs targeting the internal ribosonial entry site
Kanda, T
Zhang, B
Kusov, Y
Yokosuka, O
Gauss-Müller, V
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 330 (04) : 1217 - 1223
[6] SiRNAs do not induce RNA-dependent transcriptional silencing of retrovirus in human cells
Haraguchi, Takeshi
Mizutani, Taketoshi
Yamamichi, Nobutake
Ito, Taiji
Minoguchi, Shigeru
Iba, Hideo
FEBS LETTERS, 2007, 581 (25) : 4949 - 4954
[7] VIRsiRNApred: a web server for predicting inhibition efficacy of siRNAs targeting human viruses
Qureshi, Abid
Thakur, Nishant
Kumar, Manoj
JOURNAL OF TRANSLATIONAL MEDICINE, 2013, 11
[8] VIRsiRNApred: a web server for predicting inhibition efficacy of siRNAs targeting human viruses
Abid Qureshi
Nishant Thakur
Manoj Kumar
Journal of Translational Medicine, 11
[9] Development of Lipoprotein(a) siRNAs for Mechanism of Action Studies in Non-Human Primate Models of Atherosclerosis
Marija Tadin-Strapps
Michael Robinson
Lauretta Le Voci
Lori Andrews
Satya Yendluri
Stephanie Williams
Steve Bartz
Douglas G. Johns
Journal of Cardiovascular Translational Research, 2015, 8 : 44 - 53
[10] Development of Lipoprotein(a) siRNAs for Mechanism of Action Studies in Non-Human Primate Models of Atherosclerosis
Tadin-Strapps, Marija
Robinson, Michael
Le Voci, Lauretta
Andrews, Lori
Yendluri, Satya
Williams, Stephanie
Bartz, Steve
Johns, Douglas G.
JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH, 2015, 8 (01) : 44 - 53

← 1 2 3 4 5 →