Thrombin Augments LPS-Induced Human Endometrial Endothelial Cell Inflammation via PAR1 Activation

被引:20
|
作者
Mhatre, Mohak V. [1 ,3 ]
Potter, Julie A. [1 ]
Lockwood, Charles J. [2 ]
Krikun, Graciela [1 ]
Abrahams, Vikki M. [1 ]
机构
[1] Yale Univ, Sch Med, Dept Obstet Gynecol & Reprod Sci, New Haven, CT USA
[2] Univ S Florida, Morsani Coll Med, Dept Obstet & Gynecol, Tampa, FL USA
[3] Tufts Med Ctr, Dept Obstet & Gynecol, Boston, MA USA
关键词
Endothelial; infection; inflammation; pregnancy; thrombin; TOLL-LIKE RECEPTOR; FETAL MEMBRANES; INTERLEUKIN-8; EXPRESSION; CYTOKINE SIGNALING-3; PRETERM LABOR; MECHANISMS; INFECTION; SUPPRESSOR; ABRUPTION; TERM;
D O I
10.1111/aji.12517
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Problem Risk factors for preterm birth include placental abruption, giving rise to excessive decidual thrombin, and intrauterine bacterial infection. Human endometrial endothelial cells (HEECs) express Toll-like receptors (TLRs), and infection-derived agonists trigger HEECs to generate specific inflammatory responses. As thrombin, in addition to inducing coagulation, can contribute to inflammation, its effect on HEEC inflammatory responses to the TLR4 agonist, bacterial lipopolysaccharide (LPS), was investigated. Method of study HEECs were pre-treated with or without thrombin or specific protease-activated receptor (PAR) agonists, followed by treatment with or without LPS. Supernatants were measured for cytokines and chemokines by ELISA and multiplex analysis. Results Thrombin significantly and synergistically augmented LPS-induced HEEC secretion of interleukin (IL)-6, IL-8, granulocyte colony-stimulating factor (G-CSF), and growth-regulated oncogene-alpha (GRO-alpha), and significantly augmented monocyte chemotactic protein (MCP)-1, tumor necrosis factor-alpha (TNF-alpha), and vascular endothelial growth factor (VEGF) secretion additively. Similar to thrombin, a PAR1 agonist synergistically augmented the LPS-induced HEEC secretion of inflammatory IL-6, IL-8, G-CSF, and GRO-alpha. Conclusion Thrombin, via PAR1 activation, synergistically augments LPS-induced HEEC production of chemokines involved in immune cell recruitment and survival, suggesting a mechanism by which intrauterine abruption and bacterial infection may together be associated with an aggravated uterine inflammatory response.
引用
收藏
页码:29 / 37
页数:9
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