New Isoflavonoid Glycosides and Related Constituents from Astragali Radix (Astragalus membranaceus) and Their Inhibitory Activity on Nitric Oxide Production

被引：110

作者：

Zhang, Li-Jie ^{[1
]}

Liu, Hui-Kang ^{[1
]}

Hsiao, Ping-Chun ^{[1
,2
]}

Kuo, Li-Ming Yang ^{[1
]}

Lee, I-Jung ^{[1
]}

Wu, Tian-Shung ^{[3
,4
]}

Chiou, Wen-Fei ^{[1
]}

Kuo, Yao-Haur ^{[1
,5
]}

机构：

[1] Natl Res Inst Chinese Med, Taipei 112, Taiwan

[2] Taipei Med Univ, Grad Inst Pharmacognosy, Taipei 110, Taiwan

[3] China Med Univ & Hosp, Chinese Med Res & Dev Ctr, Taichung 404, Taiwan

[4] China Med Univ & Hosp, Dept Pharm, Taichung 404, Taiwan

[5] China Med Univ, Grad Inst Integrated Med, Taichung 404, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 04期

关键词：

Astragali radix; nitric oxide production; isoflavonoids; astragalasides; HAIRY ROOT CULTURES; CYCLOARTANE TRITERPENE GLYCOSIDES; PLANT-TISSUE CULTURES; IN-VITRO; VAR; MONGHOLICUS; PTEROCARPAN; MACROPHAGE; EXPRESSION; CELLS;

D O I：

10.1021/jf103610j

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Twenty-four secondary metabolites, induding 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (-)-methylinissolin 3-O-beta-D-(6'-acetyl)-glucoside (1), (-)-methylinissolin 3-O-beta-D-{6'-[(E)-but-2-enoyl]}-glucoside (2), and calycosin 7-O-beta-D-(6"-acetyl)-glucoside (3) have been identified as new compounds on the basis of spectroscopic analysis; (-)-methylinissolin 3-O-beta-D-glucoside (4) was isolated from the natural products for the first time. The nitric oxide (NO) production inhibitory activity of the major compounds has been assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. To identify A. membranaceus, a fingerprint method was developed by using a high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method. Furthermore, characteristic peaks for the 11 major compounds in the chromatogram were unambiguously confirmed.

引用

页码：1131 / 1137

页数：7