New Isoflavonoid Glycosides and Related Constituents from Astragali Radix (Astragalus membranaceus) and Their Inhibitory Activity on Nitric Oxide Production

被引：110
作者：
Zhang, Li-Jie [1 ]
Liu, Hui-Kang [1 ]
Hsiao, Ping-Chun [1 ,2 ]
Kuo, Li-Ming Yang [1 ]
Lee, I-Jung [1 ]
Wu, Tian-Shung [3 ,4 ]
Chiou, Wen-Fei [1 ]
Kuo, Yao-Haur [1 ,5 ]
机构：
[1] Natl Res Inst Chinese Med, Taipei 112, Taiwan
[2] Taipei Med Univ, Grad Inst Pharmacognosy, Taipei 110, Taiwan
[3] China Med Univ & Hosp, Chinese Med Res & Dev Ctr, Taichung 404, Taiwan
[4] China Med Univ & Hosp, Dept Pharm, Taichung 404, Taiwan
[5] China Med Univ, Grad Inst Integrated Med, Taichung 404, Taiwan
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2011年 / 59卷 / 04期
关键词：
Astragali radix; nitric oxide production; isoflavonoids; astragalasides; HAIRY ROOT CULTURES; CYCLOARTANE TRITERPENE GLYCOSIDES; PLANT-TISSUE CULTURES; IN-VITRO; VAR; MONGHOLICUS; PTEROCARPAN; MACROPHAGE; EXPRESSION; CELLS;
D O I：
10.1021/jf103610j
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
Twenty-four secondary metabolites, induding 16 isoflavonoids, 7 astragalasides, and 1 benzoquinone, have been isolated from the roots of Astragalus membranaceus (Astragali radix). Among these isolated isoflavonoids, (-)-methylinissolin 3-O-beta-D-(6'-acetyl)-glucoside (1), (-)-methylinissolin 3-O-beta-D-{6'-[(E)-but-2-enoyl]}-glucoside (2), and calycosin 7-O-beta-D-(6"-acetyl)-glucoside (3) have been identified as new compounds on the basis of spectroscopic analysis; (-)-methylinissolin 3-O-beta-D-glucoside (4) was isolated from the natural products for the first time. The nitric oxide (NO) production inhibitory activity of the major compounds has been assessed in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. To identify A. membranaceus, a fingerprint method was developed by using a high-performance liquid chromatography-evaporative light scattering detector (HPLC-ELSD) method. Furthermore, characteristic peaks for the 11 major compounds in the chromatogram were unambiguously confirmed.
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收藏
页码：1131 / 1137
页数：7
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