Immunohistochemical investigations on Brucella ceti-infected, neurobrucellosis-affected striped dolphins (Stenella coeruleoalba)

被引：6

作者：

Di Francesco, Gabriella ^{[1
]}

Petrini, Antonio ^{[1
]}

D'Angelol, Anna Rita ^{[1
]}

Di Renzo, Ludovica ^{[1
]}

Luciani, Mirella ^{[1
]}

Di Febo, Tiziana ^{[1
]}

Ruggieri, Enzo ^{[1
]}

Petrella, Antonio ^{[2
]}

Grattarola, Carla ^{[3
]}

Iulini, Barbara ^{[3
]}

Matteucci, Osvaldo ^{[1
]}

Lucifora, Giuseppe ^{[4
]}

Sierra, Eva ^{[5
]}

Fernandez, Antonio ^{[5
]}

Stuffier, Roberto Giacominelli ^{[6
]}

Angelucci, Clotilde ^{[6
]}

Baffoni, Marina ^{[6
]}

Di Guardo, Giovanni ^{[6
]}

Tittarelli, Manuela ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Abruzzo & Molise G, I-64100 Teramo, Italy

[2] Ist Zooprofilatt Sperimentale Puglia & Basilicata, Foggia, Italy

[3] Ist Zooprofilatt Sperimentale Piemonte Liguria &, Turin, Italy

[4] Ist Zooprofilatt Sperimentale Mezzogiorno, Portici, NA, Italy

[5] Univ Las Palmas Gran Canaria, Vet Sch, Inst Anim Hlth, Las Palmas Gran Canaria, Spain

[6] Univ Teramo, Fac Vet Med, Teramo, Italy

来源：

VETERINARIA ITALIANA | 2019年 / 55卷 / 04期

关键词：

Brucella ceti; Immunohistochemistry; Neurobrucellosis; Stenella coeruleoalba; SEROLOGICAL DIAGNOSIS; STRAINS;

D O I：

10.12834/VetIt.1920.10224.2

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Bacteria of the genus Brucella cause brucellosis, an infectious disease common to humans as well as to terrestrial and aquatic mammals. Since 1994 several cases of Brucella spp. infection have been reported in marine mammals worldwide. While sero-epidemiological data suggest that Brucella spp. infection is widespread globally, detecting Brucella spp.associated antigens by immunohistochemistry (IHC) in tissues from infected animals is often troublesome. The present study was aimed at investigating, by means of IHC based upon the utilization of an anti-Brucella LPS monoclonal antibody (MAb), the central nervous system (CNS) immunoreactivity shown by B. ceti-infected, neurobrucellosis-affected striped dolphins. The aforementioned MAb, previously characterized by means of ELISA and Western Blotting techniques, was able to immunohistochemically detect smooth brucellae both within the CNS from B. ceti-infected striped dolphins and within a range of tissues from Brucella spp.infected domestic ruminants. In conclusion, the results of the present study are of relevance both from the B. ceti infection's diagnostic and pathogenetic standpoints.

引用

页码：363 / 367

页数：5

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