Microfluidics for cell separation

被引:515
作者
Bhagat, Ali Asgar S. [2 ]
Bow, Hansen [3 ]
Hou, Han Wei [2 ,4 ]
Tan, Swee Jin [4 ]
Han, Jongyoon [1 ,2 ,3 ]
Lim, Chwee Teck [2 ,4 ,5 ]
机构
[1] MIT, Dept Biol Engn, Cambridge, MA 02139 USA
[2] SMART Ctr, BioSyM IRG, Singapore 117543, Singapore
[3] MIT, Dept Elect Engn & Comp Sci, Cambridge, MA 02139 USA
[4] Natl Univ Singapore, Div Bioengn, Singapore 117576, Singapore
[5] Natl Univ Singapore, Dept Mech Engn, Singapore 117576, Singapore
关键词
RED-BLOOD-CELLS; ON-A-CHIP; CONTINUOUS PARTICLE SEPARATION; PINCHED FLOW FRACTIONATION; ELECTROPHORETIC SEPARATION; PERIPHERAL-BLOOD; GENE-EXPRESSION; SINGLE CELLS; LIVING CELLS; CANCER-CELLS;
D O I
10.1007/s11517-010-0611-4
中图分类号
TP39 [计算机的应用];
学科分类号
081203 ; 0835 ;
摘要
The need for efficient cell separation, an essential preparatory step in many biological and medical assays, has led to the recent development of numerous microscale separation techniques. This review describes the current state-of-the-art in microfluidics-based cell separation techniques. Microfluidics-based sorting offers numerous advantages, including reducing sample volumes, faster sample processing, high sensitivity and spatial resolution, low device cost, and increased portability. The techniques presented are broadly classified as being active or passive depending on the operating principles. The various separation principles are explained in detail along with popular examples demonstrating their application toward cell separation. Common separation metrics, including separation markers, resolution, efficiency, and throughput, of these techniques are discussed. Developing efficient microscale separation methods that offering greater control over cell population distribution will be important in realizing true point-of-care (POC) lab-on-a-chip (LOC) systems.
引用
收藏
页码:999 / 1014
页数:16
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