Exogenous Biliverdin Improves the Function of Lung Grafts From Brain Dead Donors in Rats

被引:15
作者
Wang, J. [1 ]
Zhou, H. -C. [1 ]
Pan, P. [1 ]
Zhang, N. [1 ]
Li, W. -Z. [1 ]
机构
[1] Harbin Med Univ, Dept Anesthesiol, Affiliated Hosp 2, Harbin 150086, Heilongjiang Pr, Peoples R China
关键词
HEME OXYGENASE-1 INDUCTION; ISCHEMIA-REPERFUSION; IN-VIVO; INJURY; ACTIVATION; BILIRUBIN; REDUCTASE; PROTECTS; LIVER; TRANSPLANTATION;
D O I
10.1016/j.transproceed.2010.01.076
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. Biliverdin, a product of heme oxygenase-1 (HO-1), ameliorates the posttransplant functions of heart, kidney, and liver. In this study, we investigated the effects of biliverdin on lung grafts from brain dead (BD) rat donors. Methods. Male Wistar rats were randomly divided into 3 groups. The sham group (n = 7), did not undergo BD. Both donor and recipient rats in the BD biliverdin group (n = 8) were injected with biliverdin (35 mg/kg in 1 mL) intraperitoneally after confirmed BD and transplantation. In the BD group (n = 8), both donor and recipient rats received the same volume of saline (35 mg/kg in 1 mL) as the BD biliverdin group. All donor rats were observed for 1.5 hours before undergoing lung transplantation. Two hours after transplantation, we obtained blood and lung graft samples. Results. Biliverdin reversed the aggravation of Pao(2) in recipients, reduced the grafts wet/dry ratio, decreased the severity of lung injury measured by histologic examination, reduced serum tumor necrosis factor-alpha and interleukin-8 levels and inhibited myeloperoxidase activity (MPO) in the grafts. Furthermore, it significantly decreased malonaldehyde levels and increased superoxide dismutase levels. Biliverdin reduced cell apoptosis, activated protein expression of biliverdin reductase, and inhibited expression of HO-1 and nuclear factor (NF)-kappa B in lung grafts. Conclusion. Biliverdin exerts protective effects on lung grafts from BD donors through anti-inflammatory, antioxidant, and anti-apoptotic mechanisms.
引用
收藏
页码:1602 / 1609
页数:8
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