Evaluation of an Integrated Ultrafiltration/Solid Phase Extraction Process for Purification of Oligomeric Grape Seed Procyanidins

被引：9

作者：

Gutierrez-Docio, Alba ^{[1
]}

Almodovar, Paula ^{[1
,2
]}

Moreno-Fernandez, Silvia ^{[1
]}

Manuel Silvan, Jose ^{[3
]}

Martinez-Rodriguez, Adolfo J. ^{[3
]}

Luis Alonso, Gonzalo ^{[4
]}

Prodanov, Marin ^{[1
]}

机构：

[1] Univ Autonoma Madrid, Inst Invest Ciencias Alimentac CIAL, Dept Prod & Characterizat Novel Foods, Funct Food Ingredients Grp,CSIC,CEI, C Nicolas Cabrera 9,Campus Cantoblanco, Madrid 28049, Spain

[2] Pharmact Biotech Prod SL, Parque Cient Madrid, Madrid 28049, Spain

[3] Univ Autonoma Madrid, Inst Invest Ciencias Alimentac CIAL, Dept Biotechnol & Food Microbiol, Microbiol & Food Biocatalysis Grp,CSIC,CEI, C Nicolas Cabrera 9,Campus Cantoblanco, Madrid 28049, Spain

[4] Univ Castilla La Mancha, ETSI Agronomos & Montes, Catedra Quim Agr, Avda Espana S-N, Albacete 02071, Spain

来源：

MEMBRANES | 2020年 / 10卷 / 07期

关键词：

grape seed extract; purification; tangential-flow pressure-driven membrane ultrafiltration; solid-phase extraction; oligomeric procyanidins; CONDENSED TANNINS; PROANTHOCYANIDINS; ANTIBACTERIAL; DOXORUBICIN; SEPARATION; NUTRITION; DIMERS; ACID; L;

D O I：

10.3390/membranes10070147

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effectiveness of a preparative integrated ultrafiltration/solid-phase extraction (UF/SPE) process for purification of oligomeric procyanidins (OPCs) from a crude grape seed extract (GSE) was studied for the first time. The separation of OPCs from polymeric procyanidins (PPCs) by UF was very efficient. The membrane showed an acceptable filtration flux of 6 to 3.5 L/h center dot m(2)at 0.5 bar of transmembrane pressure and 95% recovery of its water flux after chemical cleaning. The process was scalable to a pilot scale. The separation of very polar and ionic species from OPCs by SPE (XAD7HP and XAD16 resins) was also very good, but both adsorbents lost their retention capacities quickly, due probably to irreversible retention of OPCs/PPCs. Even though the global purification of OPCs by the integrated UF/SPE process allowed the recovery of 24.2 g of highly purified OPCs (83% purity) from 14.4 L of crude grape seed extract, the use of these adsorbents for further purification of the OPCs was very limited.

引用

页码：1 / 15

页数：16

共 47 条

[1] [Anonymous], 1998, FREE RADICAL RES, DOI DOI 10.1080/10715769800300391
[2] [Anonymous], 1999, J CHROMATOGR A, DOI DOI 10.1016/S0021-9673(99)00281-2
[3] PHENOLICS IN ECOLOGICAL INTERACTIONS - THE IMPORTANCE OF OXIDATION
APPEL, HM
[J]. JOURNAL OF CHEMICAL ECOLOGY, 1993, 19 (07) : 1521 - 1552
[4] Efficient isolation of major procyanidin A-type dimers from peanut skins and B-type dimers from grape seeds
Appeldoorn, Maaike M.
Sanders, Mark
Vincken, Jean-Paul
Cheynier, Veronique
Le Guerneve, Christine
Hollman, Peter C. H.
Gruppen, Harry
[J]. FOOD CHEMISTRY, 2009, 117 (04) : 713 - 720
[5] Procyanidin Dimers Are Metabolized by Human Microbiota with 2-(3,4-Dihydroxyphenyl)acetic Acid and 5-(3,4-Dihydroxyphenyl)-γ-valerolactone as the Major Metabolites
Appeldoorn, Maaike M.
Vincken, Jean-Paul
Aura, Anna-Marja
Hollman, Peter C. H.
Gruppen, Harry
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (03) : 1084 - 1092
[6] Aspe E., 2015, J MED BIOENGINEERING, V4, P178, DOI DOI 10.12720/jomb.4.3.178-183
[7] Bagchi D, 1997, RES COMMUN MOL PATH, V95, P179
[8] Modulatory role of grape seed extract on age-related oxidative DNA damage in central nervous system of rats
Balu, M
Sangeetha, P
Murali, G
Panneerselvam, C
[J]. BRAIN RESEARCH BULLETIN, 2006, 68 (06) : 469 - 473
[9] Clarification and concentration of pomegranate juice (Punica granatum L.) using membrane processes
Cassano, Alfredo
Conidi, Carmela
Drioli, Enrico
[J]. JOURNAL OF FOOD ENGINEERING, 2011, 107 (3-4) : 366 - 373
[10] Safety evaluation, anti-oxidative and anti-inflammatory effects of subchronically dietary supplemented high dosing grape seed powder (GSP) to healthy rat
Charradi, Kamel
Mahmoudi, Mohamed
Bedhiafi, Takwa
Jebari, Khawla
El May, Michele Veronique
Limam, Ferid
Aouani, Ezzedine
[J]. BIOMEDICINE & PHARMACOTHERAPY, 2018, 107 : 534 - 546

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