Absolute calibration of optical tweezers including aberrations

被引：19
作者：
Dutra, R. S. [1 ,2 ,3 ]
Viana, N. B. [2 ,3 ]
Maia Neto, P. A. [2 ,3 ]
Nussenzveig, H. M. [2 ,3 ]
机构：
[1] Inst Fed Educ Ciencia & Tecnol, BR-26600000 Paracambi, RJ, Brazil
[2] Univ Fed Rio de Janeiro, LPO COPEA, Inst Ciencias Biomed, BR-21941590 Rio De Janeiro, RJ, Brazil
[3] Univ Fed Rio de Janeiro, Inst Fis, BR-21941972 Rio De Janeiro, RJ, Brazil
来源：
APPLIED PHYSICS LETTERS | 2012年 / 100卷 / 13期
关键词：
IMAGE;
D O I：
10.1063/1.3699273
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We extend a previous proposal for absolute calibration of optical tweezers by including optical setup aberrations into the first-principles theory, with no fitting parameters. Astigmatism, the dominant term, is determined from images of the focused laser spot. Correcting it can substantially increase stiffness. Comparison with experimental results yields agreement within error bars for a broad range of bead sizes and trap heights, as well as different polarizations. Absolute calibration is established as a reliable and practical method for applications and design of optical tweezers systems. (C) 2012 American Institute of Physics. [http://dx.doi.org/10.1063/1.3699273]
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页数：3
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