bZIP transcription factor OsbZIP52/RISBZ5: a potential negative regulator of cold and drought stress response in rice

被引：198
作者：
Liu, Citao [1 ]
Wu, Yanbin [2 ]
Wang, Xiping [1 ,2 ]
机构：
[1] Beijing Normal Univ, Key Lab Gene Engn Drug & Biotechnol, Key Lab Cell Proliferat & Regulat, Minist Educ,Coll Life Sci, Beijing 100875, Peoples R China
[2] Weiming Kaituo Agr Biotech Co Ltd, Natl Ctr Mol Crop Design, Beijing 100085, Peoples R China
来源：
PLANTA | 2012年 / 235卷 / 06期
基金：
中国国家自然科学基金;
关键词：
Abiotic stress; bZIP; Transcription factor; G-box; Transgenic rice (Oryza sativa); SALT TOLERANCE; PHOSPHATASE GENE; G-BOX; PROTEIN; EXPRESSION; ACTIVATOR; ELEMENT; FAMILY; DEFENSE; HETERODIMERIZATION;
D O I：
10.1007/s00425-011-1564-z
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
OsbZIP52/RISBZ5 is a member of the basic leucine zipper (bZIP) transcription factor (TF) family in rice (Oryza sativa) isolated from rice (Zhonghua11) panicles. Expression of the OsbZIP52 gene was strongly induced by low temperature (4A degrees C) but not by drought, PEG, salt, or ABA. The subcellular localization of OsbZIP52-GFP in onion (Allium cepa) epidermis cells revealed that OsbZIP52 is a nuclear localized protein. A transactivation assay in yeast demonstrated that OsbZIP52 functions as a transcriptional activator and can specifically bind to the G-box promoter motif. In a yeast two-hybrid (Y-2-H) experiment, OsbZIP52 was able to form homodimeric complexes. Rice plants overexpressing OsbZIP52 showed significantly increased sensitivity to cold and drought stress. Real-time PCR analysis revealed that some abiotic stress-related genes, such as OsLEA3, OsTPP1, Rab25, gp1 precursor, beta-gal, LOC_Os05g11910 and LOC_Os05g39250, were down-regulated in OsbZIP52 overexpression lines. These results suggest that OsbZIP52/RISBZ5 could function as a negative regulator in cold and drought stress environments.
引用
收藏
页码：1157 / 1169
页数：13
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