Dynamics of microbial community and fermentation quality during ensiling of sterile and nonsterile alfalfa with or without Lactobacillus plantarum inoculant

被引：212

作者：

Yang, Lili ^{[1
]}

Yuan, Xianjun ^{[1
]}

Li, Junfeng ^{[1
]}

Dong, Zhihao ^{[1
]}

Shao, Tao ^{[1
]}

机构：

[1] Nanjing Agr Univ, Coll Agrograssland Sci, Inst Ensiling & Proc Grass, Nanjing 210095, Jiangsu, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2019年 / 275卷

基金：

中国国家自然科学基金;

关键词：

Alfalfa; gamma-Ray irradiation; Ensiling; Lactobacillus plantarum; High throughput sequencing; LACTIC-ACID BACTERIA; AEROBIC STABILITY; CHEMICAL-COMPOSITION; SILAGE; STERILIZATION; DEGRADATION; GRASS; CORN;

D O I：

10.1016/j.biortech.2018.12.067

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

To reveal the mechanism of the survival and adaption of inoculated Lactobacillus plantarum during ensiling. Alfalfa was ensiled directly (A1), after gamma-ray irradiation (A0), and after inoculation of the sterile (A0L) or fresh alfalfa (A1L) with Lactobacillus plantarum. The A0L had the higher lactic acid content and lower pH than that in A1L from 3 days of ensiling. Pediococcus was the dominant microbes in A1 silage, followed by Enterococcus and Lactobacillus, while Lactobacillus in A1L outnumbered all other genera at 3 d. In A0L silage, the relative abundance of Lactobacillus increased to 99.13% at day 3. It indicated that Lactobacillus could dominated the fermentation of inoculated silages regardless of the gamma-ray irradiation, although there was a short lag period for irradiated alfalfa.

引用

页码：280 / 287

页数：8

共 35 条

[21] DEGRADATION OF FRUCTANS BY EPIPHYTIC AND INOCULATED LACTIC-ACID BACTERIA AND BY PLANT ENZYMES DURING ENSILAGE OF NORMAL AND STERILE HYBRID RYEGRASS [J].

MERRY, RJ ;

WINTERS, AL ;

THOMAS, PI ;

MULLER, M ;

MULLER, T .

JOURNAL OF APPLIED BACTERIOLOGY, 1995, 79 (06) :583-591

[22] Assessing the fermentation quality and microbial community of the mixed silage of forage soybean with crop corn or sorghum [J].

Ni, Kuikui ;

Zhao, Jingyun ;

Zhu, Baoge ;

Su, Rina ;

Pan, Yi ;

Ma, Junkui ;

Zhou, Guoan ;

Tao, Yong ;

Liu, Xiaorong ;

Zhong, Jin .

BIORESOURCE TECHNOLOGY, 2018, 265 :563-567

[23] Effects of lactic acid bacteria and molasses additives on the microbial community and fermentation quality of soybean silage [J].

Ni, Kuikui ;

Wang, Fangfang ;

Zhu, Baoge ;

Yang, Junxiang ;

Zhou, Guoan ;

Pan, Yi ;

Tao, Yong ;

Zhong, Jin .

BIORESOURCE TECHNOLOGY, 2017, 238 :706-715

[24] Comparative microbiota assessment of wilted Italian ryegrass, whole crop corn, and wilted alfalfa silage using denaturing gradient gel electrophoresis and next-generation sequencing [J].

Ni, Kuikui ;

Tang Thuy Minh ;

Tran Thi Minh Tu ;

Tsuruta, Takeshi ;

Pang, Huili ;

Nishino, Naoki .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (04) :1385-1394

[25] Effects of ensiling forage soybean (Glycine max (L.) Merr.) with or without bacterial inoculants on the fermentation characteristics, aerobic stability and nutrient digestion of the silage by Damara rams [J].

Nkosi, B. D. ;

Meeske, R. ;

Langa, T. ;

Motiang, M. D. ;

Modiba, S. ;

Mkhize, N. R. ;

Groenewald, I. B. .

SMALL RUMINANT RESEARCH, 2016, 134 :90-96

[26] Bacterial diversity and composition of alfalfa silage as analyzed by Illumina MiSeq sequencing: Effects of Escherichia coli O157:H7 and silage additives [J].

Ogunade, I. M. ;

Jiang, Y. ;

Cervantes, A. A. Pech ;

Kim, D. H. ;

Oliveira, A. S. ;

Vyas, D. ;

Weinberg, Z. G. ;

Jeong, K. C. ;

Adesogan, A. T. .

JOURNAL OF DAIRY SCIENCE, 2018, 101 (03) :2048-2059

[27] Meta-analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows [J].

Oliveira, Andre S. ;

Weinberg, Zwi G. ;

Ogunade, Ibukun M. ;

Cervantes, Andres A. P. ;

Arriola, Kathy G. ;

Jiang, Yun ;

Kim, Donghyeon ;

Li, Xujiao ;

Goncalves, Mariana C. M. ;

Vyas, Diwakar ;

Adesogan, Adegbola T. .

JOURNAL OF DAIRY SCIENCE, 2017, 100 (06) :4587-4603

[28] Natural populations of lactic acid bacteria associated with silage fermentation as determined by phenotype, 16S ribosomal RNA and recA gene analysis [J].

Pang, Huili ;

Qin, Guangyong ;

Tan, Zhongfang ;

Li, Zongwei ;

Wang, Yanping ;

Cai, Yimin .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2011, 34 (03) :235-241

[29] Silage review: Foodborne pathogens in silage and their mitigation by silage additives [J].

Queiroz, O. C. M. ;

Ogunade, I. M. ;

Weinberg, Z. ;

Adesogan, A. T. .

JOURNAL OF DAIRY SCIENCE, 2018, 101 (05) :4132-4142

[30]

THOMAS TA, 1977, J SCI FOOD AGR, V28, P639, DOI 10.1002/jsfa.2740280711

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