Generic Immunoassay of Quinolones: Production and Characterization of Anti-pefloxacin Antibodies as Broad Selective Receptors

Polyclonal antibodies were raised against pefloxacin, and their specificity to different quinolones was investigated and validated. The results of the competitive indirect enzyme-linked immunosorbent assay (ci-ELISA) demonstrated that the mice anti-pefloxacin antibody could efficiently bind with at least nine quinolones, and to six of them, the cross-reactivity was calculated higher than 65%. Exploiting these antibodies as receptors, six quinolones in fortified eel samples were effectively detected by both ci-ELISA and dot immunogold filtration assay, in which the least detection limit was estimated as a parts per thousand currency signaEuro parts per thousand 10 mu g/kg and a parts per thousand currency signaEuro parts per thousand 20 mu g/kg, respectively. These results fit well with molecular modeling studies based on field-overlapping and allow us to suggest possible applications of anti-pefloxacin antibody as a new broad selective receptor for generic assay of quinolones.