Fluorescence-quenching-based enzyme-activity assay by using photon upconversion

被引：99

作者：

Rantanen, Terhi ^{[1
]}

Jaervenpaeae, Marja-Leena ^{[1
]}

Vuojola, Johanna ^{[1
]}

Kuningas, Katri ^{[1
]}

Soukka, Tero ^{[1
]}

机构：

[1] Univ Turku, Dept Biotechnol, FIN-20520 Turku, Finland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 20期

关键词：

analytical methods; enzyme activity; luminescence; phosphors; sensors;

D O I：

10.1002/anie.200705861

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Switch the lights off! A novel sensitive enzyme-activity assay combines the advantageous features of upconverting phosphors (UCPs) and fluorescence quenching. The new assay principle allows the use of fluorescent particulate labels in a quenching-based assay despite the fact that it is not possible to achieve adequate quenching efficiency of particle fluorescence directly. Infrared excitation enables even the use of colorful samples. (Figure Presented) © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3811 / 3813

页数：3

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