This paper employs the concept of Protestant Hierotopy to explore the spiritual roots of Dutch Golden Age Painting. Hierotopic methodology focuses on the creation of sacred spaces as a form of human creativity. Though the Reformation may have done away with ecclesiastical sacred spaces, it introduced a new kind of hierotopy in their place: a sacralization of the whole of Creation, with a focus on human environments. Protestant admiration for nature was imbued with religious feelings, while cleanliness and domesticity came to be seen as closely akin to holiness. In this paper I interpret Dutch Golden Age Painting as an iconography of this new form of sacrality. I argue that what we find in this art ought to be understood, not as a purely descriptive, objective realism conceived for its own sake, but rather as a passionate or even sacred naturalism motivated by admiration for God's marvelous creation and enhanced by a Protestant sense of co-working with the Creator.