Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens

被引：45

作者：

Bezstarosti, Karel ^{[1
]}

Lamers, Mart M. ^{[2
]}

Doff, Wouter A. S. ^{[1
]}

Wever, Peter C. ^{[3
]}

Thai, Khoa T. D. ^{[4
]}

van Kampen, Jeroen J. A. ^{[2
]}

Haagmans, Bart L. ^{[2
]}

Demmers, Jeroen A. A. ^{[1
]}

机构：

[1] Erasmus MC, Prote Ctr, Rotterdam, Netherlands

[2] Erasmus MC, Virosci Dept, Rotterdam, Netherlands

[3] Jeroen Bosch Hosp, Shertogenbosch, Netherlands

[4] Star Shl Med Diagnost Ctr, Rotterdam, Netherlands

来源：

PLOS ONE | 2021年 / 16卷 / 11期

关键词：

SAMPLES;

D O I：

10.1371/journal.pone.0259165

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics.

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