Magnesium lithospermate B decreases [Ca2+]1 in endothelial cells by inhibiting K+ currents

被引：5

作者：

Zhang, Haifei ^{[1
]}

Zhang, Jie ^{[1
]}

Zha, Ruopeng ^{[1
]}

Hu, Miaomiao ^{[1
]}

Wang, Yiping ^{[1
]}

机构：

[1] Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2011年 / 650卷 / 01期

关键词：

Endothelial cell; Magnesium lithospermate B; K+ current; Membrane potential; SALVIAE-MILTIORRHIZAE RADIX; ION CHANNELS; ACID-B; CALCIUM; EXTRACT;

D O I：

10.1016/j.ejphar.2010.09.028

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Magnesium lithospermate B (MLB) is a hydrophilic active component of Salviae miltiorrhizae Radix Studies have shown that MLB affected intracellular calcium ([Ca2+](1)) but the underlying mechanism was unclear yet The present work was aimed to investigate the underlying mechanism of MLB affecting [Ca2+](1) in endothelial cells (ECs) Isolated mesentery arteries were employed to test the involvement of L-Ca2+ channel [Ca2+](1) was measured in ECs loaded with Fluo 3 Membrane potential and membrane currents were recorded in ECs using patch-clamp techniques Results showed that MLB did not inhibit Ca2+ influx via L-Ca2+ channel in isolated mesenteric arteries However MLB decreased [Ca2+](1) in a concentration-dependent manner in ECs MLB depolarized the membrane potential of ECs and inhibited K+ currents These results demonstrated that MLB decreased [Ca2+](1) by inhibiting K+ currents and depolarizing membrane potential in ECs (C) 2010 Elsevier B V All rights reserved

引用

页码：285 / 289

页数：5

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