An experimental model for the study of craniofacial deformities

被引：7

作者：

Costa, Andre de Mendonca

Kobayashi, Gerson Shigeru ^{[1
]}

Bueno, Daniela Franco ^{[1
]}

Martins, Marilia Trierveiler ^{[2
]}

Ferreira, Marcus de Castro ^{[3
]}

Passos-Bueno, Maria Rita ^{[1
]}

Alonso, Nivaldo ^{[3
]}

机构：

[1] Univ Sao Paulo, Inst Biosci, BR-09500900 Sao Paulo, Brazil

[2] Univ Sao Paulo, Dept Oral Pathol, Sch Dent, BR-09500900 Sao Paulo, Brazil

[3] FMUSP, Div Plast Surg, Dept Surg, Sao Paulo, Brazil

来源：

ACTA CIRURGICA BRASILEIRA | 2010年 / 25卷 / 03期

关键词：

Bone Regeneration; Craniofacial Abnormalities; Parietal Bone; Rats; MARROW STROMAL CELLS; BONE-MARROW; CRANIAL DEFECTS; STEM-CELLS; REPAIR; RECONSTRUCTION; REGENERATION; OSTEOGENESIS; DURA;

D O I：

10.1590/S0102-86502010000300008

中图分类号：

R61 [外科手术学];

学科分类号：

摘要：

Purpose: To develop an experimental surgical model in rats for the study of craniofacial abnormalities. Methods: Full thickness calvarial defects with 10x10-mm and 5x8-mm dimensions were created in 40 male NIS Wistar rats, body weight ranging from 320 to 420 g. The animals were equally divided into two groups. The periosteum was removed and dura mater was left intact. Animals were killed at 8 and 16 weeks postoperatively and cranial tissue samples were taken from the defects for histological analysis. Results: Cranial defects remained open even after 16 weeks postoperatively. Conclusion: The experimental model with 5x8-mm defects in the parietal region with the removal of the periosteum and maintenance of the integrity of the dura mater are critical and might be used for the study of cranial bone defects in craniofacial abnormalities.

引用

页码：264 / 268

页数：5

共 19 条

[1] Applications of a mouse model of calvarial healing: Differences in regenerative abilities of juveniles and adults [J].