Involvement of the calcium-sensing receptor in mineral trioxide aggregate induced osteogenic gene expression in murine MC3T3-E1 cells

被引：7
作者：
Yasukawa, Takuya [1 ]
Hayashi, Makoto [1 ]
Tanabe, Natsuko [2 ]
Tsuda, Hiromasa [2 ]
Suzuki, Yusuke [1 ]
Kawato, Takayuki [3 ]
Suzuki, Naoto [2 ]
Maeno, Masao [3 ]
Ogiso, Bunnai [1 ]
机构：
[1] Nihon Univ, Sch Dent, Dept Endodont, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
[2] Nihon Univ, Sch Dent, Dept Biochem, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
[3] Nihon Univ, Sch Dent, Dept Oral Hlth Sci, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
来源：
DENTAL MATERIALS JOURNAL | 2017年 / 36卷 / 04期
关键词：
Mineral trioxide aggregate; Calcium-sensing receptor; Osteogenesis; IN-VITRO; PARATHYROID-HORMONE; ION RELEASE; DIFFERENTIATION; PROLIFERATION; CHEMOTAXIS; ACTIVATION; APOPTOSIS; PROROOT; ROLES;
D O I：
10.4012/dmj.2016-313
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-El. cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.
引用
收藏
页码：469 / 475
页数：7
相关论文
共 50 条
  • [41] Effects of different oxygen concentrations on the proliferation, survival, migration, and osteogenic differentiation of MC3T3-E1 cells
    Liu, Haixin
    Yang, Minsheng
    Wu, Guofeng
    Yang, Lianjun
    Cao, Yanlin
    Liu, Chun
    Tan, Zhiwen
    Jin, Yanglei
    Guo, Jiasong
    Zhu, Lixin
    CONNECTIVE TISSUE RESEARCH, 2019, 60 (03) : 240 - 253
  • [42] Surface modifications by gas plasma control osteogenic differentiation of MC3T3-E1 cells
    Barradas, Ana M. C.
    Lachmann, Kristina
    Hlawacek, Gregor
    Frielink, Cathelijne
    Truckenmoller, Roman
    Boerman, Otto C.
    van Gastel, Raoul
    Garritsen, Henk
    Thomas, Michael
    Moroni, Lorenzo
    van Blitterswijk, Clemens
    de Boer, Jan
    ACTA BIOMATERIALIA, 2012, 8 (08) : 2969 - 2977
  • [43] (Zn, Ni)-ferrite nanoparticles for promoted osteogenic differentiation of MC3T3-E1 cells
    Mohan, Harshavardhan
    Karthi, Natesan
    Sathya, Pavithra Muthukumar
    Ramalingam, Vaikundamoorthy
    Thimmarayan, Srivalli
    Hossain, Mohammad Amjad
    Aravinthan, Adithan
    Shin, Taeho
    JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY, 2022, 111 : 454 - 463
  • [44] Dexamethasone induces caspase activation in murine osteoblastic MC3T3-E1 cells
    Chua, CC
    Chua, BHL
    Chen, ZY
    Landy, C
    Hamdy, RC
    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2003, 1642 (1-2): : 79 - 85
  • [45] Osteogenic effect of Drynariae rhizoma extracts and Naringin on MC3T3-E1 cells and an induced rat alveolar bone resorption model
    Chen, Li-li
    Lei, Li-hong
    Ding, Pei-hui
    Tang, Qi
    Wu, Yan-min
    ARCHIVES OF ORAL BIOLOGY, 2011, 56 (12) : 1655 - 1662
  • [46] Suppressive effect of dexamethasone on TIMP-1 production involves murine osteoblastic MC3T3-E1 cell apoptosis
    Xie, Hui
    Tang, Ling-Li
    Luo, Xiang-Hang
    Wu, Xi-Yu
    Wu, Xian-Ping
    Zhou, Hou-De
    Yuan, Ling-Qing
    Liao, Er-Yuan
    AMINO ACIDS, 2010, 38 (04) : 1145 - 1153
  • [47] Cannabinoid receptor 1 regulates ERK and GSK-3β-dependent glucocorticoid inhibition of osteoblast differentiation in murine MC3T3-E1 cells
    Wu, Re-Wen
    Lin, Tzu-Ping
    Ko, Jih-Yang
    Yeh, Da-Wei
    Chen, Ming-Wen
    Ke, Huei-Ching
    Wu, Shin-Long
    Wang, Feng-Sheng
    BONE, 2011, 49 (06) : 1255 - 1263
  • [48] Diazoxide protects against hydrogen peroxide-induced toxicity in the osteoblastic MC3T3-E1 cells
    Choi, Eun Mi
    Kim, Gun-Hee
    Lee, Yong Soo
    EUROPEAN JOURNAL OF PHARMACOLOGY, 2009, 624 (1-3) : 45 - 50
  • [49] Effect of autophagy on irradiation-induced damage in osteoblast-like MC3T3-E1 cells
    Li, Rui
    Yang, Wenke
    Hu, Xurui
    Zhou, Dan
    Huang, Ke
    Wang, Chenwei
    Li, Yi
    Liu, Bin
    MOLECULAR MEDICINE REPORTS, 2020, 22 (04) : 3473 - 3481
  • [50] Effects of Silicon on Osteoblast Activity and Bone Mineralization of MC3T3-E1 Cells
    Kim, Eun-Jin
    Bu, So-Young
    Sung, Mi-Kyung
    Choi, Mi-Kyeong
    BIOLOGICAL TRACE ELEMENT RESEARCH, 2013, 152 (01) : 105 - 112
12345