Involvement of the calcium-sensing receptor in mineral trioxide aggregate induced osteogenic gene expression in murine MC3T3-E1 cells

被引：7
作者：
Yasukawa, Takuya [1 ]
Hayashi, Makoto [1 ]
Tanabe, Natsuko [2 ]
Tsuda, Hiromasa [2 ]
Suzuki, Yusuke [1 ]
Kawato, Takayuki [3 ]
Suzuki, Naoto [2 ]
Maeno, Masao [3 ]
Ogiso, Bunnai [1 ]
机构：
[1] Nihon Univ, Sch Dent, Dept Endodont, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
[2] Nihon Univ, Sch Dent, Dept Biochem, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
[3] Nihon Univ, Sch Dent, Dept Oral Hlth Sci, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
来源：
DENTAL MATERIALS JOURNAL | 2017年 / 36卷 / 04期
关键词：
Mineral trioxide aggregate; Calcium-sensing receptor; Osteogenesis; IN-VITRO; PARATHYROID-HORMONE; ION RELEASE; DIFFERENTIATION; PROLIFERATION; CHEMOTAXIS; ACTIVATION; APOPTOSIS; PROROOT; ROLES;
D O I：
10.4012/dmj.2016-313
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-El. cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.
引用
收藏
页码：469 / 475
页数：7
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