Involvement of the calcium-sensing receptor in mineral trioxide aggregate induced osteogenic gene expression in murine MC3T3-E1 cells

被引：7
|
作者：
Yasukawa, Takuya [1 ]
Hayashi, Makoto [1 ]
Tanabe, Natsuko [2 ]
Tsuda, Hiromasa [2 ]
Suzuki, Yusuke [1 ]
Kawato, Takayuki [3 ]
Suzuki, Naoto [2 ]
Maeno, Masao [3 ]
Ogiso, Bunnai [1 ]
机构：
[1] Nihon Univ, Sch Dent, Dept Endodont, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
[2] Nihon Univ, Sch Dent, Dept Biochem, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
[3] Nihon Univ, Sch Dent, Dept Oral Hlth Sci, Chiyoda Ku, 1-8-13 Kanda Surugadai, Tokyo 1018310, Japan
来源：
DENTAL MATERIALS JOURNAL | 2017年 / 36卷 / 04期
关键词：
Mineral trioxide aggregate; Calcium-sensing receptor; Osteogenesis; IN-VITRO; PARATHYROID-HORMONE; ION RELEASE; DIFFERENTIATION; PROLIFERATION; CHEMOTAXIS; ACTIVATION; APOPTOSIS; PROROOT; ROLES;
D O I：
10.4012/dmj.2016-313
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Mineral trioxide aggregate (MTA) has excellent biocompatibility as well as bioactivity, including an ability to induce osteoblast differentiation. We examined the effects of the calcium-sensing receptor (CaSR) on osteogenic gene expression induced by MTA. MC3T3-El. cells were cultured with or without (control) MTA. The expression levels of Runx2, type I collagen, and CaSR genes were analyzed by real-time polymerase chain reaction and their products were measured using enzyme-linked immunosorbent assays. The levels were increased significantly in cells exposed to MTA compared with control. Next, MC3T3-E1 cells were cultured with MTA and EGTA (a calcium chelator), because calcium ions were released continuously from MTA into the culture. Expression levels were decreased to control levels by MTA plus EGTA. NPS2143 (a CaSR antagonist) also reduced MTA-induced gene expression. These results suggest that MTA induced osteogenic gene expressions of Runx2 and type I collagen via CaSR in MC3T3-E1 cells.
引用
收藏
页码：469 / 475
页数：7
相关论文
共 50 条
  • [1] Identification of a Calcium-sensing Receptor in Human Dental Pulp Cells That Regulates Mineral Trioxide Aggregate-induced Mineralization
    Chen, Yanhuo
    Gao, Yan
    Tao, Yifan
    Lin, Dongjia
    An, Shaofeng
    JOURNAL OF ENDODONTICS, 2019, 45 (07) : 907 - 916
  • [2] Orexins Facilitates Osteogenic Differentiation of MC3T3-E1 Cells
    Han, Xuesong
    Zhou, Jicheng
    Peng, Wei
    IUBMB LIFE, 2018, 70 (07) : 633 - 641
  • [3] Gene Expression in MC3T3-E1 Cells Treated with Diclofenac and Methylprednisolone
    Lehmann, Tomasz P.
    Iwanczyk-Skalska, Ewa
    Harasymczuk, Jerzy
    Jagodzinski, Pawel P.
    Glowacki, Maciej
    GENES, 2023, 14 (01)
  • [4] Effect of progesterone on apoptosis of murine MC3T3-E1 osteoblastic cells
    Wang, Qing-Ping
    Xie, Hui
    Yuan, Ling-Qing
    Luo, Xiang-Hang
    Li, Hui
    Wang, Dan
    Meng, Ping
    Liao, Er-Yuan
    AMINO ACIDS, 2009, 36 (01) : 57 - 63
  • [5] Osteogenic role of endosomal chloride channels in MC3T3-E1 cells
    Wang, Huan
    Huo, Na
    Li, Feifei
    Fu, Shanmin
    Xue, Yang
    Yang, Ting
    Wen, Xuan
    Ding, Yin
    Duan, Xiaohong
    MOLECULAR AND CELLULAR BIOCHEMISTRY, 2010, 342 (1-2) : 191 - 199
  • [6] Titania nanotube delivery fetal bovine serum for enhancing MC3T3-E1 activity and osteogenic gene expression
    Peng, Jing
    Zhang, Xinming
    Li, Zhaoyang
    Liu, Yunde
    Yang, Xianjin
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2015, 56 : 438 - 443
  • [7] Functional expression of 5-HT2A receptor in osteoblastic MC3T3-E1 cells
    Hirai, Takao
    Kaneshige, Kota
    Kurosaki, Teruko
    Nishio, Hiroaki
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2010, 396 (02) : 278 - 282
  • [8] Effects of Continuous Passaging on Mineralization of MC3T3-E1 Cells with Improved Osteogenic Culture Protocol
    Yan, Xiang-Zhen
    Yang, Wanxun
    Yang, Fang
    Kersten-Niessen, Monique
    Jansen, John A.
    Both, Sanne K.
    TISSUE ENGINEERING PART C-METHODS, 2014, 20 (03) : 198 - 204
  • [9] Genome-wide analysis for nanofiber induced global gene expression profile: A study in MC3T3-E1 cells by RNA-Seq
    Wang, Peiyan
    Meng, Xinyue
    Xue, Junqiang
    Fan, Changjiang
    Wang, Jianxun
    COLLOIDS AND SURFACES B-BIOINTERFACES, 2023, 223
  • [10] ZIP1 and Zinc Inhibits Fluoride-Induced Apoptosis in MC3T3-E1 Cells
    Xu, Shihong
    Yang, Yongliang
    Han, Shumei
    Wu, Zonghui
    BIOLOGICAL TRACE ELEMENT RESEARCH, 2014, 159 (1-3) : 399 - 409
12345