Changes of Androgen Receptor and Insulin-Like Growth Factor-1 in LNCaP Prostate Cancer Cells Treated with Sex Hormones and Flutamide

被引：0

作者：

Tong, Da Yue ^{[1
]}

Wen, Xing Qiao ^{[2
]}

Jin, Yi ^{[2
]}

Liu, Zhuo Wei ^{[3
]}

Sun, Hong Yu ^{[1
]}

Zhou, Fang Jian ^{[3
]}

Wu, Xin Yao ^{[1
]}

机构：

[1] Sun Yat Sen Univ, Zhongshan Sch Med, Dept Forens Med, Guangzhou, Guangdong, Peoples R China

[2] Sun Yat Sen Univ, Affiliated Hosp 3, Guangzhou, Guangdong, Peoples R China

[3] Sun Yat Sen Univ, Canc Ctr, Guangzhou, Guangdong, Peoples R China

来源：

ASIAN PACIFIC JOURNAL OF CANCER PREVENTION | 2010年 / 11卷 / 06期

关键词：

Androgen receptor; DHT; insulin-like growth factor-1; LNCaP cells; real-time PCR; INDEPENDENCE; PROGRESSION; STATISTICS; EXPRESSION;

D O I：

暂无

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Changes of androgen receptor (AR) and insulin-like growth factor-1 (IGF-1) were investigated in LNCaP cells treated with 5 alpha-dihydrotestosterone (DHT), estrone and flutamide. Real-time PCR, immunocytochemistry and western blotting were used to detect the expression of AR and IGF-1 in the presence or absence of various kinase inhibitors. Low concentrations of DHT, estrone and flutamide increased the expression of AR and IGF-1, especially estrone, with concentration and time dependence. With DHT and flutamide, there was a significant alteration in AR expression (p<0.001). The results indicated expression of AR and IGF-1 genes to be influenced by DHT, estrone and flutamide in LNCaP cells, regulated by multiple signal pathways.

引用

页码：1805 / 1809

页数：5

共 19 条

[1] Insulin-like growth factors and prostate cancer [J].

Djavan, B ;

Waldert, M ;

Seitz, C ;

Marberger, M .

WORLD JOURNAL OF UROLOGY, 2001, 19 (04) :225-233

[2] Non-steroidal antiandrogens act as AF-1 agonists under conditions of high androgen-receptor expression [J].

Fuse, Hiroaki ;

Korenaga, Shigeru ;

Sakari, Matomo ;

Hiyama, Takashi ;

Ito, Takeshi ;

Kimura, Koichi ;

Kato, Shigeaki .

PROSTATE, 2007, 67 (06) :630-637

[3] Androgens and prostate cancer: Biology, pathology and hormonal therapy [J].

Galbraith, SM ;

Duchesne, GM .

EUROPEAN JOURNAL OF CANCER, 1997, 33 (04) :545-554

[4] Role of Cyclic AMP Response Element-Binding Protein in Insulin-like Growth Factor-I Receptor Up-regulation by Sex Steroids in Prostate Cancer Cells [J].

Genua, Marco ;

Pandini, Giuseppe ;

Sisci, Diego ;

Castoria, Gabriella ;

Maggiolini, Marcello ;

Vigneri, Riccardo ;

Belfiore, Antonino .

CANCER RESEARCH, 2009, 69 (18) :7270-7277

[5] Cancer statistics, 2001 [J].

Greenlee, RT ;

Hill-Harmon, MB ;

Murray, T ;

Thun, M .

CA-A CANCER JOURNAL FOR CLINICIANS, 2001, 51 (01) :15-36

[6] Cancer statistics, 2003 [J].

Jemal, A ;

Murray, T ;

Samuels, A ;

Ghafoor, A ;

Ward, E ;

Thun, MJ .

CA-A CANCER JOURNAL FOR CLINICIANS, 2003, 53 (01) :5-26

[7] The insulin-like growth factor system and cancer [J].

LeRoith, D ;

Roberts, CT .

CANCER LETTERS, 2003, 195 (02) :127-137

[8]

Lv T, 2007, MED J WUHAN U, V28, P181

[9]

Mikami K, 2009, ASIAN PAC J CANCER P, V10, P57

[10]

Naiki T, 2009, ASIAN PAC J CANCER P, V10, P71

← 1 2 →