Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation

被引：32
作者：
Fu, Jie
Munro, James B. [2 ]
Blanchard, Scott C. [2 ]
Frank, Joachim [1 ,3 ]
机构：
[1] Columbia Univ, Dept Biochem & Mol Biophys, Howard Hughes Med Inst, New York, NY 10032 USA
[2] Weill Cornell Med Coll, Dept Physiol & Biophys, New York, NY 10065 USA
[3] Columbia Univ, Dept Biol Sci, New York, NY 10027 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2011年 / 108卷 / 12期
基金：
美国国家卫生研究院;
关键词：
MESSENGER-RNA; HYBRID STATE; SINGLE-PARTICLE; L1; STALK; DYNAMICS; MOVEMENT; BINDING; SPACE; RECONSTRUCTION; CONFORMATION;
D O I：
10.1073/pnas.1101503108
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
mRNA-tRNA translocation is a central and highly regulated process during translational elongation. Along with the mRNA, tRNA moves through the ribosome in a stepwise fashion. Using cryoelectron microscopy on ribosomes with a P-loop mutation, we have identified novel structural intermediates likely to exist transiently during translocation. Our observations suggest a mechanism by which the rate of translocation can be regulated.
引用
收藏
页码：4817 / 4821
页数：5
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