Gastroentric cancers detection by Raman spectroscopy of human serum

To investigate the spectral specialities of stomach cancer serum for diagnosis, fluorescence and Raman spectra of normal, stomach cancer (both before and after operation), esophagus cancer and atrophic gastritis sera were measured in the visible region in this study. All spectra except esophagus cancer were characterized by three sharp peaks (A, B and C). The intensity of each peak was different in different spectrum. After samples were radiated by laser, fluorescence weakened along with red shift of its band center, and spectral changes of normal and stomach cancer (after operation) cases were different from other samples. It was also observed that spectral changes of atrophic gastritis were very similar with stomach cancer (such as the red shift of fluorescence peak is more than 12nm) after radiated by laser, however, there are still some distinctions that can be used to differentiate them from each other. A notable difference is that the relative intensity of peak C excited by 488.0nm is higher than excited by 514.5nm in spectrum of stomach cancer, whereas lower in other cases. We utilized it as a criterion and got an accuracy of 80.77% in stomach cancer detection.